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PRODUCT CODE: ET1610-50

Asparagine synthetase Recombinant Rabbit Monoclonal Antibody [SC67-07] (ET1610-50)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of Asparagine synthetase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-50, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.<br /> Positive control: <br /> Lane 1: K562 cell lysate<br /> Lane 2: Human skeletal muscle tissue lysate
  • Western blot analysis of Asparagine synthetase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-50, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.<br /> Positive control: <br /> Lane 1: K562 cell lysate<br /> Lane 2: Human skeletal muscle tissue lysate
  • Western blot analysis of Asparagine synthetase on human lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-50, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
  • ICC staining of Asparagine synthetase in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-50, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of Asparagine synthetase in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-50, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of Asparagine synthetase in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-50, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Asparagine synthetase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-50, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of Asparagine synthetase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-50, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: K562 cell lysate
Lane 2: Human skeletal muscle tissue lysate

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Asparagine synthetase Recombinant Rabbit Monoclonal Antibody [SC67-07] (ET1610-50)

Immunogen

Synthetic peptide within human asparagine synthetase aa 510-550.

Host

Rabbit

Positive Control

K562, SW480, Hela, SH-SY-5Y, mouse stomach tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SC67-07

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

61 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

P08243

PROTEIN NAME

Asparagine synthetase

SYNONYMS

asnS antibody; ASNS_HUMAN antibody; ASNSD antibody; Asparagine synthetase [glutamine-hydrolyzing] antibody; Asparagine synthetase antibody; Cell cycle control protein TS11 antibody; Glutamine dependent asparagine synthetase 3 antibody; Glutamine dependent asparagine synthetase antibody; Glutamine hydrolyzing antibody; Glutamine-dependent asparagine synthetase antibody; OTTHUMP00000024510 antibody; OTTHUMP00000204938 antibody; OTTHUMP00000204939 antibody; OTTHUMP00000204940 antibody; OTTHUMP00000204941 antibody; OTTHUMP00000204942 antibody; TS11 antibody; TS11 cell cycle control protein antibody

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Glutamine-hydrolyzing asparagine synthetase is also commonly designated cell cycle control protein TS11. Asparagine synthetase plays an important role in the amino-acid biosynthesis pathway and is also important for L-asparagine biosynthesis. Via the L-glutamine route, it is involved in the synthesis of L-asparaine from L-aspartate. The protein contains one asparagine synthetase domain and one type-2 glutamine amidotransferase domain. The cell-cycle regulated gene encoding for asparagine synthetase, ts11, is necessary for G1 progression.