Aryl hydrocarbon Receptor Recombinant Rabbit Monoclonal Antibody [JM34-10] (ET1703-11)
Catalog# ET1703-11
Rabbit Monoclonal to Aryl hydrocarbon Receptor
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WB
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Human
Lane 1: HepG2 cell lysate
Lane 2: A549 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 96 kDa
Observed band size: 105 kDa
Exposure time: 2 minutes 37 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-11) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Aryl hydrocarbon Receptor Recombinant Rabbit Monoclonal Antibody [JM34-10] (ET1703-11)
Immunogen
Synthetic peptide within human aryl hydrocarbon receptor aa 735-775 / 848.
Host
Rabbit
Positive Control
HepG2 cell lysate, A549 cell lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM34-10
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:2,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 96 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Aryl hydrocarbon Receptor
SYNONYMS
Ah receptor antibody;AhR antibody;AHR_HUMAN antibody;Aromatic hydrocarbon receptor antibody;Aryl hydrocarbon receptor antibody;Aryl hydrocarbon receptor precursor antibody;bHLHe76 antibody;Class E basic helix loop helix protein 76 antibody;Class E basic helix-loop-helix protein 76 antibody;HGNC:348 antibody
TISSUE SPECIFICITY
Expressed in all tissues tested including blood, brain, heart, kidney, liver, lung, pancreas and skeletal muscle. Expressed in retinal photoreceptors.
POST-TRANSLATIONAL MODIFICATION
Mono-ADP-ribosylated, leading to inhibit transcription activator activity of AHR.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus.
FUNCTION
The aryl hydrocarbon receptor (also known as AhR, AHR, ahr, ahR, or dioxin receptor) is a protein that in humans is encoded by the AHR gene. The aryl hydrocarbon receptor is a transcription factor that regulates gene expression. It was originally thought to function primarily as a sensor of xenobiotic chemicals and also as the regulator of enzymes such as cytochrome P450s that metabolize these chemicals. The most notable of these xenobiotic chemicals are aromatic (aryl) hydrocarbons from which the receptor derives its name. In addition to regulating metabolism enzymes, the AhR has roles in regulating immunity, stem cell maintenance, and cellular differentiation. The aryl hydrocarbon receptor is a member of the family of basic helix-loop-helix transcription factors. AHR binds several exogenous ligands such as natural plant flavonoids, polyphenolics and indoles, as well as synthetic polycyclic aromatic hydrocarbons and dioxin-like compounds. AhR is a cytosolic transcription factor that is normally inactive, bound to several co-chaperones. Upon ligand binding to chemicals such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), the chaperones dissociate resulting in AhR translocating into the nucleus and dimerizing with ARNT (AhR nuclear translocator), leading to changes in gene transcription.
CITATIONS
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Duan Zhenzhen et al
The Protective Effect of IDO1 Inhibition in Aβ-Treated Neurons and APP/PS1 Mice
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WB
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Rat
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Citation
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