PRODUCT CODE: HA600003

ARL13B Mouse Monoclonal Antibody [A4E4] (HA600003)

Applications

  • WB

REACTIVITY

  • Human

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+
Western blot analysis of ARL13B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600003, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse  IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: 293T cell lysate<br />
Lane 2: HL-60 cell lysate
  • Western blot analysis of ARL13B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600003, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse  IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: 293T cell lysate<br />
Lane 2: HL-60 cell lysate
Western blot analysis of ARL13B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600003, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: 293T cell lysate
Lane 2: HL-60 cell lysate

Applications

  • WB

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

ARL13B Mouse Monoclonal Antibody [A4E4] (HA600003)

Immunogen

Synthetic peptide within human arl13b aa 370-428.

Host

Mouse

Positive Control

293T cell lysate, HL-60 cell lysate.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A4E4

PROPERTIES

Form

Liquid

Storage Condition

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G affinity purified.

MOLECULAR WEIGHT

Predicted band size: 49 kDa.

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

TARGET

UNIPROT #

PROTEIN NAME

ARL13B

SYNONYMS

ADP ribosylation factor like 13B antibody; ADP ribosylation factor like 2 like 1 antibody; ADP-ribosylation factor-like protein 13B antibody; ADP-ribosylation factor-like protein 2-like 1 antibody; AR13B_HUMAN antibody; arl13b antibody; ARL2-like protein 1 antibody; ARL2L1 antibody; JBTS8 antibody

SUBCELLULAR LOCATION

Cell membrane, Cell projection, Cilium, Membrane.

FUNCTION

Cilium-specific protein required to control the microtubule-based, ciliary axoneme structure. May act by maintaining the association between IFT subcomplexes A and B. Binds GTP but is not able to hydrolyze it; the GTPase activity remains unclear. Required to pattern the neural tube. Involved in cerebral cortex development: required for the initial formation of a polarized radial glial scaffold, the first step in the construction of the cerebral cortex, by regulating ciliary signaling. Regulates the migration and placement of postmitotic interneurons in the developing cerebral cortex. May regulate endocytic recycling traffic; however, additional evidence is required to confirm these data.