Liver Arginase Recombinant Rabbit Monoclonal Antibody [PD00-05] (HA721147)
Catalog# HA721147
Rabbit Monoclonal to Arginase-1
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WB
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IHC-P
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IF-Tissue
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Human
Lysates/proteins at 20 µg/Lane.
Predicted band size: 35 kDa
Observed band size: 37 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721147) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Liver Arginase Recombinant Rabbit Monoclonal Antibody [PD00-05] (HA721147)
Immunogen
Synthetic peptide within human liver arginase (c terminal)
Host
Rabbit
Positive Control
Human liver tissue lysates, human liver tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
PD00-05
RRID
APPLICATION DILUTION
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WB
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1:2,000
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IHC-P
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1:1,000-1:5,000
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IF-Tissue
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1:500-1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 35 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Arginase-1
SYNONYMS
A I antibody;Al antibody;ARG 1 antibody;arg1 antibody;ARGI1_HUMAN antibody;Arginase 1 antibody;Arginase liver antibody;Arginase type I antibody;Arginase, liver antibody;Arginase-1 antibody;Arginase1 antibody;Liver type arginase antibody;Liver-type arginase antibody;Type I arginase antibody
SUBCELLULAR LOCATION
Cytoplasm, Cytoplasmic granule.
FUNCTION
Arginase-1 is a key urea cycle metalloenzyme that has demonstrated expression in normal human liver with a high degree of specificity. Hepatocellular carcinoma (HCC) is the most common primary malignant tumor of the liver accounting for an estimated 70%-85% of total liver cancers worldwide. Diagnostic pitfalls exist in the morphologic distinction of HCC from other hepatocellular and non-hepatocellular lesions. In difficult or equivocal cases, the application of immunohistochemical (IHC) panels has been shown to aid in the distinction of benign and malignant liver lesions. In sections of normal liver, anti-arginase-1 produced strong, diffuse cytoplasmic reactivity in all hepatocytes throughout the lobule. In some cases, patchy nuclear reactivity is also evident in hepatocytes along with the cytoplasmic reactivity. Reactivity is not observed in bile duct epithelial cells, sinusoidal endothelial cells, Kupffer cells, or vascular endothelial cells. In sections of HCC, anti-arginase-1 produces cytoplasmic or cytoplasmic plus nuclear reactivity.
CITATIONS
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HMGB1‐associated necroptosis and Kupffer cells M1 polarization underlies remote liver injury induced by intestinal ischemia/reperfusion in rats
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IF-tissue
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Mouse
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Citation
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