Liver Arginase Recombinant Rabbit Monoclonal Antibody [SY09-06] (ET1605-8)
Catalog# ET1605-8TR
Rabbit Monoclonal to ARG1
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WB
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Human
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Mouse
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Rat
Lane 1: Human liver tissue lysate
Lane 2: Mouse liver tissue lysate
Lane 3: Rat liver tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 35 kDa
Observed band size: 37 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-8) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Liver Arginase Recombinant Rabbit Monoclonal Antibody [SY09-06] (ET1605-8)
Immunogen
Synthetic peptide within human arg1 aa 20-60.
Host
Rabbit
Positive Control
Human liver tissue lysate, Mouse liver tissue lysate, Rat liver tissue lysate, THP-1 treated with 185ng/mL TPA for 54 hours add 20ng/mL hIFN-γ and 100ng/mL LPS for 48 hours cell lysate, RAW264.7 treated with 20ng/mL mIL-4 and 10ng/mL mIL-13 for 24 hours cell lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SY09-06
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:2,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 35 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
ARG1
SYNONYMS
arg1 antibody;ARGI1_HUMAN antibody;Arginase-1 antibody;Liver-type arginase antibody;Type I arginase antibody
SEQUENCE SIMILARITIES
Belongs to the arginase family.
TISSUE SPECIFICITY
Within the immune system initially reported to be selectively expressed in granulocytes (polymorphonuclear leukocytes [PMNs]). Also detected in macrophages mycobacterial granulomas. Expressed in group2 innate lymphoid cells (ILC2s) during lung disease.
SUBCELLULAR LOCATION
Cytoplasm,Cytoplasmic granule.
FUNCTION
Arginase I (also designated liver-type arginase), which is expressed almost exclusively in the liver, catalyzes the conversion of arginine to ornithine and urea. The human arginase I gene, which maps to chromosome 6q23, encodes a 322 amino acid protein. Arginase I exists as a homotrimeric protein and contains a binuclear manganese cluster. Arginase II catalyzes the same reaction as arginase I, but differs in its tissue specificity and subcellular location. Specifically, arginase II localizes to the mitochondria. Arginase II is expressed in non-hepatic tissues, with the highest levels of expression in the kidneys, but, unlike arginase I, is not expressed in liver. The human arginase II gene, which maps to chromosome 14q24.1-q24.3, encodes a 354 amino acid protein. In addition, arginase II contains a putative amino-terminal mitochondrial localization sequence.
CITATIONS
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Liang Lu,et al
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WB
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Mouse
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Citation
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Zhaoyan Chen,et al
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WB
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Mouse
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Han Liu , Haiyang Guo , Yuehan Wu , et al
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WB
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Human
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Niu Yuzhen,et al
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WB
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IHC
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Mouse
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Citation
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Liang, L., Xu, W., Shen, A., Fu, X., Cen, H., Wang, S., Lin, Z., Zhang, L., Lin, F., Zhang, X., Zhou, N., Chang, J., Chen, Z. S., Li, C., & Yu, X.
Inhibition of YAP1 activity ameliorates acute lung injury through promotion of M2 macrophage polarization
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WB
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Mouse
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Citation
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He Yining,et al
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WB
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Mouse
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Jianguo Xu,and Hong Tan
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WB
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Mouse
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Citation
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Wu Zhihao,et al
Exploring the Mechanism of Asiatic Acid against Atherosclerosis Based on Molecular Docking, Molecular Dynamics, and Experimental Verification
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WB
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Mouse
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Citation
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Wang, C., Wei, S., Liu, B., Wang, F., Lu, Z., Jin, M., & Wang, Y.
Maternal consumption of a fermented diet protects offspring against intestinal inflammation by regulating the gut microbiota
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WB
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Pig
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Citation
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He, J., Lin, M., Zhang, X., Zhang, R., Tian, T., Zhou, Y., Dong, W., Yang, Y., Sun, X., Dai, Y., Xu, Y., Zhang, Z., Xu, M., Lei, Q. Y., Xu, Y., & Lv, L.
TET2 is required to suppress mTORC1 signaling through urea cycle with therapeutic potential
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IHC
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WB
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Human
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Citation
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Zhang, L., Zhang, X., Liu, Y., Zhang, W., Wu, C. T., & Wang, L.
CD146+ Umbilical Cord Mesenchymal Stem Cells Exhibit High Immunomodulatory Activity and Therapeutic Efficacy in Septic Mice
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WB
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Mouse
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Citation
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Dan Wang,et al
Targeting SphK1/S1PR3 axis ameliorates sepsis-induced multiple organ injury via orchestration of macrophage polarization and glycolysis
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WB
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mice
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Citation
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