ARF5 Recombinant Rabbit Monoclonal Antibody [JE53-83] (ET7110-46)
Catalog# ET7110-46
Rabbit Monoclonal to ARF5
Tested for
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WB
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IHC-P
Reacts with
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Human
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Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
☑ Knockdown (KD)
Western blot analysis of ARF5 on different lysates with Rabbit anti-ARF5 antibody (ET7110-46) at 1/1,000 dilution.
Lane 1: A549-WT cell lysate
Lane 2: A549-KD ARF5 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 21 kDa
Observed band size: 18 kDa
Exposure time: 2 minutes 30 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-46) at 1/1,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of ARF5 on different lysates with Rabbit anti-ARF5 antibody (ET7110-46) at 1/1,000 dilution.
Lane 1: A549-WT cell lysate
Lane 2: A549-KD ARF5 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 21 kDa
Observed band size: 18 kDa
Exposure time: 2 minutes 30 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-46) at 1/1,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.