Aquaporin 4 Recombinant Rabbit Monoclonal Antibody [JE59-76] (HA721954)
Catalog# HA721954
Rabbit Monoclonal to Aquaporin 4
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WB
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IHC-P
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IF-Tissue
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Human
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Mouse
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Rat
Western blot analysis of Aquaporin 4 on different lysates with Rabbit anti-Aquaporin 4 antibody (HA721954) at 1/2,000 dilution.
Lane 1: Mouse brain tissue lysate (40 µg/Lane)
Lane 2: Mouse liver tissue lysate (negative) (40 µg/Lane)
Lane 3: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 35 kDa
Observed band size: 28/52 kDa
Exposure time: Lane 1: 31 seconds; Lane 2-3: 5 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721954) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Aquaporin 4 Recombinant Rabbit Monoclonal Antibody [JE59-76] (HA721954)
Immunogen
Synthetic peptide within human aquaporin 4 aa 274-323 / 323.
Host
Rabbit
Positive Control
Mouse brain tissue lysate, rat brain tissue lysate, human kidney tissue, human skeletal muscle tissue, mouse brain tissue, mouse hippocampus tissue, rat brain tissue, rat hippocampus tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE59-76
RRID
APPLICATION DILUTION
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WB
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1:2,000
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IHC-P
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1:2,000-1:10,000
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IF-Tissue
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1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 35 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Aquaporin 4
SYNONYMS
AQP 4 antibody;AQP-4 antibody;AQP4 antibody;AQP4_HUMAN antibody;Aquaporin type 4 antibody;Aquaporin-4 antibody;Aquaporin4 antibody;HMIWC 2 antibody;HMIWC2 antibody;Mercurial insensitive water channel antibody;Mercurial-insensitive water channel antibody;MGC22454 antibody;MIWC antibody;WCH 4 antibody;WCH4 antibody
SUBCELLULAR LOCATION
Cell membrane, Basolateral cell membrane, Endosome membrane, Cell membrane, sarcolemma, Cell projection.
FUNCTION
This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. This protein is the predominant aquaporin found in brain and has an important role in brain water homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. Additional isoforms, resulting from the use of alternative in-frame translation initiation codons, have also been described. Recent studies provided evidence for translational readthrough in this gene, and expression of C-terminally extended isoforms via the use of an alternative in-frame translation termination codon.
CITATIONS
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Hyaluronan-based hydrogel integrating exosomes for traumatic brain injury repair by promoting angiogenesis and neurogenesis
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IF-Tissue
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Rat
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Citation
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