PRODUCT CODE: ER1803-44

Apr3 Rabbit Polyclonal Antibody (ER1803-44)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of APR3 on human skin tissue lysates using anti-APR3 antibody.<br />
  Lane 1: Anti-APR3 antibody (1/500).<br />
  Lane 2: Anti-APR3 antibody, pre-incubated with the immunizaiton peptide.
  • Western blot analysis of APR3 on human skin tissue lysates using anti-APR3 antibody.<br />
  Lane 1: Anti-APR3 antibody (1/500).<br />
  Lane 2: Anti-APR3 antibody, pre-incubated with the immunizaiton peptide.
  • ICC staining APR3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining APR3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-APR3 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of MCF-7 cells with APR3 antibody at 1/100 dilution (Pink purple) compared with an unlabelled control (cells without incubation with primary antibody; Yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of APR3 on human skin tissue lysates using anti-APR3 antibody.
Lane 1: Anti-APR3 antibody (1/500).
Lane 2: Anti-APR3 antibody, pre-incubated with the immunizaiton peptide.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Apr3 Rabbit Polyclonal Antibody (ER1803-44)

Immunogen

Synthetic peptide within mouse apr3 aa 174-223 / 223.

Host

Rabbit

Positive Control

Human skin tissue lysates, A549, SH-SY-5Y, mouse liver tissue, MCF-7.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

25 kDa (Predicted band size)

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Apr3

SYNONYMS

APR-3; ATRAID; APR3; C2orf28; HSPC013; UNQ214/PRO240

TISSUE SPECIFICITY

Weakly expressed in hematopoietic cell lines.

SUBCELLULAR LOCATION

Plasma membrane, Nucleus envelope.

FUNCTION

This gene is thought to be involved in apoptosis, and may also be involved in hematopoietic development and differentiation. The use of alternative splice sites and promotors result in multiple transcript variants encoding different isoforms. Promotes osteoblast cell differentiation and terminal mineralization. Plays a role in inducing the cell cycle arrest via inhibiting CCND1 expression in all-trans-retinoic acid (ATRA) signal pathway.