PRODUCT CODE: ER1803-10

ApoER2 Rabbit Polyclonal Antibody (ER1803-10)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of ApoER2 on different lysates using anti-ApoER2 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Mouse placenta<br />
   Lane 2: SH-SY-5Y
  • Western blot analysis of ApoER2 on different lysates using anti-ApoER2 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Mouse placenta<br />
   Lane 2: SH-SY-5Y
  • ICC staining ApoER2 in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ApoER2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ApoER2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ApoER2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY-5Y cells with ApoER2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of ApoER2 on different lysates using anti-ApoER2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Mouse placenta
Lane 2: SH-SY-5Y

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

ApoER2 Rabbit Polyclonal Antibody (ER1803-10)

Immunogen

Recombinant protein within human apoer2 aa 494-722 / 963.

Host

Rabbit

Positive Control

Mouse placenat tissue, SH-SY-5Y, F9, HepG2, human placenta tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

77 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

ApoER2

SYNONYMS

APOER2 antibody; Apolipoprotein E receptor 2 antibody; low density lipoprotein receptor-related protein 8 antibody; Low-density lipoprotein receptor-related protein 8 antibody; LRP-8 antibody; LRP8 antibody; LRP8_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the LDLR family.

TISSUE SPECIFICITY

Expressed mainly in brain and placenta. Also expressed in platelets and megakaryocytic cells. Not expressed in the liver.

POST-TRANSLATIONAL MODIFICATION

O-glycosylated. Some alternatively spliced isoforms lack the O-linked sugar domain (By similarity).; Undergoes sequential, furin and gamma-secretase dependent, proteolytic processing, resulting in the extracellular release of the entire ligand-binding domain as a soluble polypeptide and in the intracellular domain (ICD) release into the cytoplasm. The gamma-secretase-dependent proteolytical processing occurs after the bulk of the extracellular domain has been shed, in a furin-dependent manner, in alternatively spliced isoforms carrying the furin cleavage site. Hypoglycosylation (mainly hypo-O-glycosylation) leads to increased extracellular cleavage, which in turn results in accelerating release of the intracellular domain (ICD) by the gamma-secretase. The resulting receptor fragment is able to inhibit Reelin signaling and in particular the Reelin-induced DAB1 phosphorylation (By similarity).; Tyrosine phosphorylated upon apoE binding.; Ubiquitinated by MYLIP leading to degradation.

SUBCELLULAR LOCATION

Cell membrane. Secreted.

FUNCTION

ApoER2 (apolipoprotein E receptor 2), also designated LRP8, is a member of the LDL receptor gene family, which includes LDL receptor, LRP, megalin, VLDLR and ApoER2. The LDL receptor family is characterized by a cluster of cysteine-rich class A repeats, epidermal growth factor (EGF)-like repeats, YWTD repeats and an O-linked sugar domain. ApoER2 is expressed in brain and placenta and has several splice variants. ApoER2 is thought to mediate the interaction of extracellular Reelin and cytosolic mDab1 (mammalian disabled protein), which activates a tyrosine kinase. This pathway regulates the migration of neurons along the radial glial fiber network during brain development.