PRODUCT CODE: EM1902-30

APOER2 Mouse Monoclonal Antibody [13B2] (EM1902-30)

Applications

  • WB

  • ICC

REACTIVITY

  • Human

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Western blot analysis of APOER2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-30, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: A549 cell lysate<br />
Lane 2: SHSY5Y cell lysate<br />
Lane 3: SKBR-3 cell lysate<br />
Lane 4: U937 cell lysate
  • Western blot analysis of APOER2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-30, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: A549 cell lysate<br />
Lane 2: SHSY5Y cell lysate<br />
Lane 3: SKBR-3 cell lysate<br />
Lane 4: U937 cell lysate
  • ICC staining of APOER2 in SKOV-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1902-30, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
Western blot analysis of APOER2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-30, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A549 cell lysate
Lane 2: SHSY5Y cell lysate
Lane 3: SKBR-3 cell lysate
Lane 4: U937 cell lysate

Applications

  • WB

  • ICC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

APOER2 Mouse Monoclonal Antibody [13B2] (EM1902-30)

Immunogen

Recombinant protein corresponding to n terminal of human apoer2.

Host

Mouse

Positive Control

A549 cell lysate, SHSY5Y cell lysate, SKBR-3 cell lysate, U937 cell lysate, SKOV-3.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

13B2

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified

MOLECULAR WEIGHT

Predicted band size: 106/78/99/87 kDa.

Isotype

IgG2a

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50

TARGET

UNIPROT #

PROTEIN NAME

APOER2

SYNONYMS

APOER2 antibody; Apolipoprotein E receptor 2 antibody; low density lipoprotein receptor-related protein 8 antibody; Low-density lipoprotein receptor-related protein 8 antibody; LRP-8 antibody; LRP8 antibody; LRP8_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the LDLR family.

TISSUE SPECIFICITY

Expressed mainly in brain and placenta. Also expressed in platelets and megakaryocytic cells. Not expressed in the liver.

POST-TRANSLATIONAL MODIFICATION

O-glycosylated. Some alternatively spliced isoforms lack the O-linked sugar domain (By similarity).; Undergoes sequential, furin and gamma-secretase dependent, proteolytic processing, resulting in the extracellular release of the entire ligand-binding domain as a soluble polypeptide and in the intracellular domain (ICD) release into the cytoplasm. The gamma-secretase-dependent proteolytical processing occurs after the bulk of the extracellular domain has been shed, in a furin-dependent manner, in alternatively spliced isoforms carrying the furin cleavage site. Hypoglycosylation (mainly hypo-O-glycosylation) leads to increased extracellular cleavage, which in turn results in accelerating release of the intracellular domain (ICD) by the gamma-secretase. The resulting receptor fragment is able to inhibit Reelin signaling and in particular the Reelin-induced DAB1 phosphorylation (By similarity).; Tyrosine phosphorylated upon apoE binding.; Ubiquitinated by MYLIP leading to degradation.

SUBCELLULAR LOCATION

Cell membrane, Membrane, Secreted.

FUNCTION

Cell surface receptor for Reelin (RELN) and apolipoprotein E (apoE)-containing ligands. LRP8 participates in transmitting the extracellular Reelin signal to intracellular signaling processes, by binding to DAB1 on its cytoplasmic tail. Reelin acts via both the VLDL receptor (VLDLR) and LRP8 to regulate DAB1 tyrosine phosphorylation and microtubule function in neurons. LRP8 has higher affinity for Reelin than VLDLR. LRP8 is thus a key component of the Reelin pathway which governs neuronal layering of the forebrain during embryonic brain development. Binds the endoplasmic reticulum resident receptor-associated protein (RAP). Binds dimers of beta 2-glycoprotein I and may be involved in the suppression of platelet aggregation in the vasculature. Highly expressed in the initial segment of the epididymis, where it affects the functional expression of clusterin and phospholipid hydroperoxide glutathione peroxidase (PHGPx), two proteins required for sperm maturation. May also function as an endocytic receptor. Not required for endocytic uptake of SEPP1 in the kidney which is mediated by LRP2 (By similarity). Together with its ligand, apolipoprotein E (apoE), may indirectly play a role in the suppression of the innate immune response by controlling the survival of myeloid-derived suppressor cells (By similarity).