AP-2 complex subunit alpha-1 Recombinant Rabbit Monoclonal Antibody [JE40-38] (HA721717)
Catalog# HA721717
Rabbit Monoclonal to AP-2 complex subunit alpha-1
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WB
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IHC-P
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Human
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Mouse
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Rat
Lane 1: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 108 kDa
Observed band size: 108 kDa
Exposure time: 10 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721717) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
AP-2 complex subunit alpha-1 Recombinant Rabbit Monoclonal Antibody [JE40-38] (HA721717)
Immunogen
Recombinant protein within human ap-2 complex subunit alpha-1 aa 1-100 / 977.
Host
Rabbit
Positive Control
Mouse brain tissue lysate, rat brain tissue lysate, human brain tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE40-38
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IHC-P
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1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 108 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
AP-2 complex subunit alpha-1
SYNONYMS
100 kDa coated vesicle protein A antibody;Adapter-related protein complex 2 alpha-1 subunit antibody;adapter-related protein complex 2 subunit alpha-1 antibody;Adaptin alpha A antibody;Adaptin alpha antibody;Adaptor protein complex AP 2 alpha 1 subunit antibody;adaptor protein complex AP 2 subunit alpha 1 antibody;Adaptor protein complex AP-2 subunit alpha-1 antibody;Adaptor protein complex AP2 alpha 1 subunit antibody;adaptor protein complex AP2 subunit alpha 1 antibody;Adaptor related protein complex 2 alpha 1 subunit antibody;Adaptor related protein complex AP 2 alpha 1 subunit antibody;Adaptor related protein complex AP2 alpha 1 subunit antibody;ADTAA antibody;Alpha A adaptin antibody;Alpha adaptin A antibody;Alpha-adaptin A antibody;alpha1 adaptin antibody;Alpha1-adaptin antibody;AP 2 alpha antibody;AP 2 complex subunit alpha 1 antibody;AP-2 complex subunit alpha-1 antibody;AP2 complex subunit alpha 1 antibody;AP2-ALPHA antibody;Ap2a1 antibody;AP2A1_HUMAN antibody;CLAPA 1 antibody;CLAPA1 antibody;Clathrin adaptor complex AP2. alpha subunit antibody;Clathrin assembly protein complex 2 alpha A large chain antibody;Clathrin assembly protein complex 2 alpha-A large chain antibody;Clathrin associated / assembly / adaptor protein large alpha 1 antibody;clathrin associated assembly adaptor protein large alpha 1 antibody;clathrin associated/assembly/adaptor protein, large, alpha 1 antibody;Plasma membrane adaptor HA 2/AP 2 adaptin alpha A subunit antibody;Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit antibody
SUBCELLULAR LOCATION
Cell membrane, Membrane, coated pit.
FUNCTION
This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined.
CITATIONS
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Yan Yuanliang,et al
Suppression of ITPKB degradation by Trim25 confers TMZ resistance in glioblastoma through ROS homeostasis
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WB
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Mouse
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Citation
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