Endothelial Cell Marker Antibody Sampler Kit (HAK21170)
Catalog# HAK21170
Reacts with
-
Human
Western blot analysis of CD45 on different lysates with Rabbit anti-CD45 antibody (HA723606) at 1/5,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: MCF7 cell lysate (negative)
Lane 3: Raji cell lysate
Lane 4: A20 cell lysate
Lane 5: C2C12 cell lysate (negative)
Lane 6: RAW234.7 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 145 kDa
Observed band size: 250 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723606) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Jurkat cell lysate
Lane 2: MCF7 cell lysate (negative)
Lane 3: Raji cell lysate
Lane 4: A20 cell lysate
Lane 5: C2C12 cell lysate (negative)
Lane 6: RAW234.7 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 145 kDa
Observed band size: 250 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723606) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.