Phospho-HER2/ErbB2 Antibody Sampler Kit (HAK21162)
Catalog# HAK21162
Reacts with
-
Human
Western blot analysis of Phospho-HER2 / ErbB2 (Y1248) on different lysates with Rabbit anti-Phospho-HER2 / ErbB2 (Y1248) antibody (HA722084) at 1/1,000 dilution.
Lane 1: SK-Br-3 cell lysate
Lane 2: SK-Br-3 starved for 4 hours add 200ng/mL EGF for 15 minutes cell lysate
Lane 3: HeLa cell lysate
Lane 4: HeLa starved for 4 hours add 200ng/mL EGF for 15 minutes cell lysate
Lane 5: HeLa starved for 4 hours add 200ng/mL EGF for 15 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 138 kDa
Observed band size: 180 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722084) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: SK-Br-3 cell lysate
Lane 2: SK-Br-3 starved for 4 hours add 200ng/mL EGF for 15 minutes cell lysate
Lane 3: HeLa cell lysate
Lane 4: HeLa starved for 4 hours add 200ng/mL EGF for 15 minutes cell lysate
Lane 5: HeLa starved for 4 hours add 200ng/mL EGF for 15 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 138 kDa
Observed band size: 180 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722084) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.