Pro-Apoptosis Bcl-2 Family Antibody Sampler Kit (HAK21159)
Catalog# HAK21159
Reacts with
-
Human
Western blot analysis of Bid on different lysates with Rabbit anti-Bid antibody (HA723593) at 1/5,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: HT-29 cell lysate
Lane 3: A549 cell lysate
Lane 4: RAW264.7 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723593) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Jurkat cell lysate
Lane 2: HT-29 cell lysate
Lane 3: A549 cell lysate
Lane 4: RAW264.7 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723593) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.