Cadherin-Catenin Antibody Sampler Kit (HAK21157)
Catalog# HAK21157
Reacts with
-
Human
Western blot analysis of N Cadherin on different lysates with Rabbit anti-N Cadherin antibody (ET1607-37) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution.
Lane 1: 293T cell lysate
Lane 2: A549 cell lysate
Lane 3: HeLa cell lysate
Lane 4: A-172 cell lysate
Lane 5: MCF7 cell lysate (negative)
Lane 6: C2C12 cell lysate
Lane 7: C6 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 100 kDa
Observed band size: 140-150 kDa
Exposure time: 2 minutes 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-37) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: 293T cell lysate
Lane 2: A549 cell lysate
Lane 3: HeLa cell lysate
Lane 4: A-172 cell lysate
Lane 5: MCF7 cell lysate (negative)
Lane 6: C2C12 cell lysate
Lane 7: C6 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 100 kDa
Observed band size: 140-150 kDa
Exposure time: 2 minutes 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-37) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.