Protein Folding and Stability Antibody Sampler Kit (HAK21156)
Catalog# HAK21156
Reacts with
-
Human
Western blot analysis of SKP2 on different lysates with Rabbit anti-SKP2 antibody (HA722654) at 1/1,000 dilution.
Lane 1: MDA-MB-231 cell lysate
Lane 2: 293T cell lysate
Lane 3: MDA-MB-468 cell lysate
Lane 4: HepG2 cell lysate
Lane 5: SK-OV-3 cell lysate
Lane 6: SH-SY5Y cell lysate
Lane 7: COS-1 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 20 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722654) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: MDA-MB-231 cell lysate
Lane 2: 293T cell lysate
Lane 3: MDA-MB-468 cell lysate
Lane 4: HepG2 cell lysate
Lane 5: SK-OV-3 cell lysate
Lane 6: SH-SY5Y cell lysate
Lane 7: COS-1 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 20 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722654) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.