Type II keratin Antibody Sampler Kit Antibody Sampler Kit (HAK21151)
Catalog# HAK21151
Reacts with
-
Human
Western blot analysis of pan Cytokeratin on different lysates with Mouse anti-pan Cytokeratin antibody (HA601138) at 1/2,000 dilution.
Lane 1: A431 cell lysate (10 µg/Lane)
Lane 2: HeLa cell lysate (10 µg/Lane)
Lane 3: MCF7 cell lysate (10 µg/Lane)
Lane 4: SK-Br-3 cell lysate (10 µg/Lane)
Lane 5: MDA-MB-231 cell lysate (10 µg/Lane)
Lane 6: MDA-MB-468 cell lysate (10 µg/Lane)
Lane 7: Human liver tissue lysate (20 µg/Lane)
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601138) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A431 cell lysate (10 µg/Lane)
Lane 2: HeLa cell lysate (10 µg/Lane)
Lane 3: MCF7 cell lysate (10 µg/Lane)
Lane 4: SK-Br-3 cell lysate (10 µg/Lane)
Lane 5: MDA-MB-231 cell lysate (10 µg/Lane)
Lane 6: MDA-MB-468 cell lysate (10 µg/Lane)
Lane 7: Human liver tissue lysate (20 µg/Lane)
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601138) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.