IAP Family Antibody Sampler Kit (HAK21148)
Catalog# HAK21148
Reacts with
-
Human
Western blot analysis of cIAP2 on different lysates with Rabbit anti-cIAP2 antibody (HA722767) at 1/2,000 dilution.
Lane 1: Raji cell lysate
Lane 2: Daudi cell lysate
Lane 3: Ramos cell lysate
Lane 4: NCI-H1299 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 68 kDa
Observed band size: 70 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722767) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Raji cell lysate
Lane 2: Daudi cell lysate
Lane 3: Ramos cell lysate
Lane 4: NCI-H1299 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 68 kDa
Observed band size: 70 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722767) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.