Annexin V Recombinant Rabbit Monoclonal Antibody [JF50-11] (ET1702-62)
Catalog# ET1702-62
Rabbit Monoclonal to Annexin V
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WB
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FC
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Human
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Mouse
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Rat
Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: Human liver tissue lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: C2C12 cell lysate
Lane 6: Mouse liver tissue lysate
Lane 7: C6 cell lysate
Lane 8: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Exposure time: 5 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-62) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Annexin V Recombinant Rabbit Monoclonal Antibody [JF50-11] (ET1702-62)
Immunogen
Synthetic peptide within human annexin v aa 281-320 / 320.
Host
Rabbit
Positive Control
HeLa cell lysate, HepG2 cell lysate, Human liver tissue lysate, NIH/3T3 cell lysate, C2C12 cell lysate, Mouse liver tissue lysate, C6 cell lysate, PC-12 cell lysate, A549 cell lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JF50-11
RRID
APPLICATION DILUTION
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WB
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 36 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Annexin V
SYNONYMS
Anchorin CII antibody;Annexin 5 antibody;Annexin A5 antibody;Annexin V antibody;Annexin-5 antibody;ANX A5 antibody;ANX5 antibody;ANXA5 antibody;ANXA5_HUMAN antibody;Calphobindin I antibody;CBP-I antibody;Endonexin II antibody;ENX2 antibody;Lipocortin V antibody;PAP I antibody;PAP-I antibody;Placental anticoagulant protein 4 antibody;Placental anticoagulant protein I antibody;PLACENTAL PROTEIN 4 antibody;Pp4 antibody;Thromboplastin inhibitor antibody;VAC-alpha antibody;Vascular anticoagulant-alpha antibody
SEQUENCE SIMILARITIES
Belongs to the annexin family.
POST-TRANSLATIONAL MODIFICATION
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
SUBCELLULAR LOCATION
Cytosol, collagen-containing extracellular matrix, endothelial microparticle, extracellular exosome, extracellular region, external side of plasma membrane, cytoplasm, focal adhesion, intracellular, membrane.
FUNCTION
The annexin family of calcium-binding proteins is composed of at least ten mammalian genes and is characterized by a conserved core domain, which binds phospholipids in a Ca2+-dependent manner, and a unique amino-terminal region, which may confer binding specificity. Annexin family members have been implicated as regulators of such diverse processes as ion flux, endocytosis and exocytosis, and cellular adhesion. For example, the crystal structure of Annexin III has suggested a hydrophilic amino-terminus with possible Ca2+ channel activity. Similarly, Annexin V has ion channel properties. Annexin IV, also referred to as endonexin, functions to regulate Cl-flux by mediating calmodulin kinase II (CaMKII) activity and Annexin V has been shown to regulate PKC activity. Annexin V is ubiquitously expressed at high levels in tissues and cells grown in tissue culture, while Annexin VIII exhibits a more limited distribution. Where co-expressed in the same tissues, Annexin VIII is often expressed at a 100-fold lower level than Annexin V. However, Annexin VIII is preferentially expressed in acute promyelocytic leukemia (APL) cells, which may relate to its role in hematopoietic cell differentiation.
CITATIONS
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Plasma-Derived Exosomes in Chronic Spontaneous Urticaria Induce the Production of Mediators by Human Mast Cells
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WB
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Human
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Citation
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