PRODUCT CODE: ET1704-49

Annexin A2 Recombinant Rabbit Monoclonal Antibody [JA42-30] (ET1704-49)

  • Recombinant

Applications

  • WB

  • IHC-P

  • ICC

  • IF

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

-
+
Western blot analysis of Annexin A2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-49, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Hela cell lysate<br />
Lane 2: human kidney tissue lysate<br />
Lane 3: mouse testis tissue lysate
  • Western blot analysis of Annexin A2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-49, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Hela cell lysate<br />
Lane 2: human kidney tissue lysate<br />
Lane 3: mouse testis tissue lysate
  • ICC staining of Annexin A2 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-49, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Annexin A2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-49, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Annexin A2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-49, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Annexin A2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-49, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of Annexin A2 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1704-49, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of Annexin A2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1704-49, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: human kidney tissue lysate
Lane 3: mouse testis tissue lysate

Applications

  • WB

  • IHC-P

  • ICC

  • IF

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Annexin A2 Recombinant Rabbit Monoclonal Antibody [JA42-30] (ET1704-49)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela cell lysate, human kidney tissue lysate, mouse testis tissue lysate, Hela, human liver tissue, human colon carcinoma tissue, human prostate tissue, K562.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA42-30

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

38 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

  • ICC/IF:1:100-1:200

  • FC:1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Annexin A2

SYNONYMS

Annexin A2 antibody; Annexin II antibody; Annexin II, heavy chain antibody; Annexin-2 antibody; ANX 2 antibody; ANX2 antibody; ANX2L4 antibody; ANXA2 antibody; ANXA2_HUMAN antibody; arylsulfatase B antibody; CAL1H antibody; Calpactin I heavy chain antibody; calpactin I heavy polypeptide (p36) antibody; Calpactin I heavy polypeptide antibody; Calpactin-1 heavy chain antibody; chromobindin 8 antibody; Chromobindin-8 antibody; Epididymis secretory protein Li 270 antibody; HEL S 270 antibody; LIP2 antibody; Lipocortin II antibody; LPC2 antibody; LPC2D antibody; p36 antibody; P36 protein antibody; PAP-IV antibody; Placental anticoagulant protein IV antibody; Protein I antibody

SEQUENCE SIMILARITIES

Belongs to the annexin family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.; ISGylated.

SUBCELLULAR LOCATION

Basement membrane. Melanosome.

FUNCTION

The annexin family of calcium-binding proteins is composed of at least ten mammalian genes and is characterized by a conserved core domain which binds phospholipids in a Ca2+-dependent manner and a unique amino-terminal region which may confer binding specificity. The interaction between these proteins and biological membranes has led to the hypothesis that they are involved in cellular trafficking processes such as endocytosis, exocytosis and cellular adhesion. Annexin I, alternatively referred to as lipocortin, has been implicated as a mediator of the anti-inflammatory response produced by glucocorticoids and as an inhibitor of cPLA2, a potent mediator of inflammation. Annexin II, also called p36, exists as a monomer or as a heterotetramer, complexed with the S-100-related protein p11. This complex is termed calpactin I. In the tetrameric form, Annexin II is an efficient substrate of PKC family and Src pp60.