ANGPTL4 Recombinant Rabbit Monoclonal Antibody [PSH07-35] (HA722823)
Catalog# HA722823
to
Tested for
-
WB
Reacts with
-
Human
-
Monkey
Western blot analysis of ANGPTL4 on different lysates with Rabbit anti-ANGPTL4 antibody (HA722823) at 1/2,000 dilution.
Lane 1: A549 cell lysate
Lane 2: SW480 cell lysate
Lane 3: THP-1 cell lysate
Lane 4: HUVEC cell lysate
Lane 5: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 45 kDa
Observed band size: 60 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722823) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A549 cell lysate
Lane 2: SW480 cell lysate
Lane 3: THP-1 cell lysate
Lane 4: HUVEC cell lysate
Lane 5: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 45 kDa
Observed band size: 60 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722823) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.