PRODUCT CODE: ET1611-41

Angiotensin II Type 2 Receptor Recombinant Rabbit Monoclonal Antibody [SN71-03] (ET1611-41)

  • Recombinant

Applications

  • WB

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Angiotensin II Type 2 Receptor on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: 293T cell lysate<br />
Lane 2: Hela cell lysate
  • Western blot analysis of Angiotensin II Type 2 Receptor on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: 293T cell lysate<br />
Lane 2: Hela cell lysate
Western blot analysis of Angiotensin II Type 2 Receptor on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: 293T cell lysate
Lane 2: Hela cell lysate

Applications

  • WB

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Angiotensin II Type 2 Receptor Recombinant Rabbit Monoclonal Antibody [SN71-03] (ET1611-41)

Immunogen

Synthetic peptide within c-terminal human angiotensin ii type 2 receptor.

Host

Rabbit

Positive Control

293T cell lysate, Hela cell lysate.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN71-03

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

41 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

TARGET

UNIPROT #

PROTEIN NAME

Angiotensin II Type 2 Receptor

SYNONYMS

AGTR 2 antibody; Agtr2 antibody; AGTR2_HUMAN antibody; angiotensin II receptor type 2 antibody; Angiotensin II type-2 receptor antibody; Angiotensin receptor 2 antibody; AT 2 antibody; AT2 antibody; ATGR 2 antibody; ATGR2 antibody; MRX 88 antibody; MRX88 antibody; Type 2 angiotensin II receptor antibody; Type-2 angiotensin II receptor antibody

SEQUENCE SIMILARITIES

Belongs to the G-protein coupled receptor 1 family.

TISSUE SPECIFICITY

In adult, highly expressed in myometrium with lower levels in adrenal gland and fallopian tube. Expressed in the cerebellum. Very highly expressed in fetal kidney and intestine.

SUBCELLULAR LOCATION

Cell membrane.

FUNCTION

Angiotensin II (Ang II) is an important physiological effector of blood pressure and volume regulation through vasoconstriction, aldosterone release, sodium uptake and thirst stimulation. Although Ang II interacts with two types of cell surface receptors, AT1 and AT2, most of the major cardiovascular effects seem to be mediated through AT1. Molecular cloning of the AT1 protein has shown it to be a member of the G protein-associated seven transmembrane protein receptor family. Ang II treatment of cells results in activation of several signal transduction pathways as evidenced by tyrosine phosphorylation of several proteins and induction of others. PLCγ is phosphorylated after 30 seconds of treatment with Angiotensin II, indicating this as an early signal transduction event. Ang II treatment also stimulates phosphorylation of Shc, FAK and MAP kinases, and induces MKP-1, indicating stimulation of growth factor pathways. Ang II stimulation through AT1 has been shown to activate the JAK/Stat pathway involving a direct interaction between JAK2 and AT1 as demonstrated by coimmunoprecipitation. The AT1 receptor has no cytoplasmic kinase domain, but is able to function as a substrate for Src kinases and has several putative phosphorylation sites.