Angiotensin II Type 1 Receptor Mouse Monoclonal Antibody [11B3] (HA600029)
Catalog# HA600029
Mouse Monoclonal to Angiotensin II Type 1 Receptor
Tested for
-
WB
-
IF-Cell
-
FC
Reacts with
-
Human
-
Mouse
-
Rat
Western blot analysis of Angiotensin II Type 1 Receptor on different lysates with Mouse anti-Angiotensin II Type 1 Receptor antibody (HA600029) at 1/500 dilution.
Lane 1: PC-12 cell lysate
Lane 2: A549 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: HeLa cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 41 kDa
Observed band size: 49 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600029) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/200,000 dilution was used for 1 hour at room temperature.
Lane 1: PC-12 cell lysate
Lane 2: A549 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: HeLa cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 41 kDa
Observed band size: 49 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600029) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/200,000 dilution was used for 1 hour at room temperature.
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