PRODUCT CODE: ET1705-6

Angiopoietin 2 Recombinant Rabbit Monoclonal Antibody [JM71-34] (ET1705-6)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Angiopoietin 2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-6, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: TF-1 cell lysate<br />
Lane 2: human liver tissue lysate<br />
Lane 3: human placenta tissue lysate<br />
Lane 4: mouse liver tissue lysate
  • Western blot analysis of Angiopoietin 2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-6, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: TF-1 cell lysate<br />
Lane 2: human liver tissue lysate<br />
Lane 3: human placenta tissue lysate<br />
Lane 4: mouse liver tissue lysate
  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Angiopoietin 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-6, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Angiopoietin 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-6, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Angiopoietin 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-6, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of Angiopoietin 2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-6, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: TF-1 cell lysate
Lane 2: human liver tissue lysate
Lane 3: human placenta tissue lysate
Lane 4: mouse liver tissue lysate

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Angiopoietin 2 Recombinant Rabbit Monoclonal Antibody [JM71-34] (ET1705-6)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

TF-1 cell lysate, human liver tissue lysate, human placenta tissue lysate, mouse liver tissue lysate, human colon carcinoma tissue, human placenta tissue, mouse liver tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM71-34

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

50 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Angiopoietin 2

SYNONYMS

AGPT 2 antibody; Agpt2 antibody; ANG 2 antibody; ANG-2 antibody; ANG2 antibody; Angiopoietin 2a antibody; Angiopoietin 2B antibody; Angiopoietin-2 antibody; Angiopoietin2 antibody; ANGP2_HUMAN antibody; ANGPT 2 antibody; Angpt2 antibody; Tie2 ligand antibody

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Tie-1 and Tie-2 (also designated Tek) are novel cell surface receptor tyrosine kinases. The extracellular domain of Tie-1 has an unusual multidomain structure consisting of a cluster of three epidermal growth factor homology motifs localized between two immunoglobulin-like loops, which are followed by three fibronectin type III repeats next to the transmembrane region. Angiopoietin-1 (Ang-1) is a secreted ligand for Tie-2. Preliminary biochemical analyses of Ang-1 reveal a potential fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus. Ang-1 is an angiogenic factor that is thought to be involved in endothelial development. A related protein, angiopoietin-2 (Ang-2), has been identified as a naturally occurring antagonist of Ang-1 activation of Tie-2. In adult tissue, Ang-2 expression seems to be restricted to sites of vascular remodeling.