Angiopoietin 1 Recombinant Rabbit Monoclonal Antibody [SN67-02] (ET1611-28)
Catalog# ET1611-28
Rabbit Monoclonal to Angiopoietin 1
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WB
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Human
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Mouse
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Rat
Lane 1: U-87 MG cell lysate
Lane 2: HeLa cell lysate
Lane 3: K-562 cell lysate
Lane 4: C6 cell lysate
Lane 5: RAW264.7 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 58 kDa
Observed band size: 65 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-28) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Angiopoietin 1 Recombinant Rabbit Monoclonal Antibody [SN67-02] (ET1611-28)
Immunogen
Synthetic peptide within human angiopoietin 1 aa 249-300 / 498.
Host
Rabbit
Positive Control
U-87 MG cell lysate, HeLa cell lysate, K-562 cell lysate, C6 cell lysate, RAW264.7 cell lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SN67-02
RRID
APPLICATION DILUTION
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WB
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 58 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Angiopoietin 1
SYNONYMS
AGP 1 antibody;AGP1 antibody;AGPT antibody;ANG 1 antibody;ANG antibody;ANG-1 antibody;ANG1 antibody;Angiopoietin-1 antibody;Angiopoietin1 antibody;ANGP1_HUMAN antibody;ANGPT 1 antibody;Angpt1 antibody;KIAA0003 antibody;OTTHUMP00000227592 antibody;OTTHUMP00000227599 antibody;OTTHUMP00000227600 antibody
POST-TRANSLATIONAL MODIFICATION
Glycosylated.
SUBCELLULAR LOCATION
Secreted.
FUNCTION
Angiopoietin 1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. During pregnancy, angiopoietins act complementary to the VEGF system and contribute to endothelial cell survival and the remodeling of vessels. Few studies have examined the role of angiopoietins in human pregnancy complications like preeclampsia and intrauterine growth restriction (IUGR).
CITATIONS
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Peng Jie,et al
Allicin in pregnancy diets modulates steroid metabolism in pregnant sows and placental sulfate metabolism promoting placental angiogenesis and fetal development
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WB
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IF
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Citation
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