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PRODUCT CODE: ET1612-56

AMPK beta 1 Recombinant Rabbit Monoclonal Antibody [SD082-06] (ET1612-56)

  • Recombinant

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of AMPK beta 1 on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-56, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of AMPK beta 1 on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-56, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Immunohistochemical analysis of paraffin-embedded human smooth muscle tissue using anti-AMPK beta 1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-56, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of AMPK beta 1 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1612-56, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of AMPK beta 1 on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-56, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

AMPK beta 1 Recombinant Rabbit Monoclonal Antibody [SD082-06] (ET1612-56)

Immunogen

Synthetic peptide within human ampk beta 1 aa 90-130.

Host

Rabbit

Positive Control

A431 cell lysates, human smooth muscle tissue, A431.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD082-06

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

38 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

Q9Y478

PROTEIN NAME

AMPK beta 1

SYNONYMS

1300015D22Rik antibody; 5'-AMP-activated protein kinase subunit beta-1 antibody; 5'-AMP-activated protein kinase beta-1 subunit antibody; AAKB1_HUMAN antibody; AMP-activated protein kinase beta subunit antibody; AMP-ACTIVATED PROTEIN KINASE, NONCATALYTIC, BETA-1 antibody; AMP-activated, noncatalytic, beta-1 antibody; AMPK antibody; AMPK beta 1 chain antibody; AMPK subunit beta-1 antibody; AMPK-BETA-1 antibody; AMPKb antibody; AU021155 antibody; E430008F22 antibody; HAMPKb antibody; MGC17785 antibody; PRKAB1 antibody; Protein kinase AMP activated non catalytic subunit beta 1 antibody; protein kinase, AMP-activated, beta 1 non-catalytic subunit antibody; protein kinase, AMP-activated, noncatalytic, beta-1 antibody

SEQUENCE SIMILARITIES

Belongs to the 5'-AMP-activated protein kinase beta subunit family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK.

SUBCELLULAR LOCATION

Cytosol, nucleoplasm, nucleus, cytoplasm, nucleotide-activated protein kinase complex.

FUNCTION

AMPK (for 5'-AMP-activated protein kinase) is a heterotrimeric complex com-prising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming bio-synthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxy-methylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The human AMPKα1 and AMPKα2 genes encode 548 amino acid and 552 amino acid proteins, respectively. Human AMPKβ1 encodes a 271 amino acid protein and human AMPKβ2 encodes a 272 amino acid protein. The human AMPKγ1 gene encodes a 331 amino acid protein. Human AMPKγ2 and AMPKγ3, which are 569 and 492 amino acid proteins, respectively, contain unique N-terminal domains and may participate directly in the binding of AMP within the AMPK complex.