PRODUCT CODE: HA600080

AMPK alpha 2 Mouse Monoclonal Antibody [A6A12] (HA600080)

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of AMPK alpha 2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600080, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Hela cell lysate<br />
Lane 2: Jurkat cell lysate<br />
Lane 3: NIH/3T3 cell lysate
  • Western blot analysis of AMPK alpha 2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600080, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Hela cell lysate<br />
Lane 2: Jurkat cell lysate<br />
Lane 3: NIH/3T3 cell lysate
  • Western blot analysis of AMPK alpha 2 on PC-12 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600080, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.
Western blot analysis of AMPK alpha 2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600080, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: Jurkat cell lysate
Lane 3: NIH/3T3 cell lysate

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

AMPK alpha 2 Mouse Monoclonal Antibody [A6A12] (HA600080)

Immunogen

Recombinant protein within human ampk alpha 2 aa 231-430.

Host

Mouse

Positive Control

Hela cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, PC-12 cell lysates.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A6A12

PROPERTIES

Form

Liquid

Storage Condition

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G affinity purified.

MOLECULAR WEIGHT

62 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500

TARGET

UNIPROT #

PROTEIN NAME

AMPK alpha 2

SYNONYMS

5'-AMP-activated protein kinase catalytic subunit alpha-2 antibody;AAPK2_HUMAN antibody;ACACA kinase antibody;Acetyl CoA carboxylase kinase antibody;Acetyl-CoA carboxylase kinase antibody;AMPK alpha 2 chain antibody;AMPK subunit alpha-2 antibody;AMPK2 antibody;AMPKa2 antibody;AMPKalpha2 antibody;HMGCR kinase antibody;Hydroxymethylglutaryl CoA reductase kinase antibody;Hydroxymethylglutaryl-CoA reductase kinase antibody;PRKAA antibody;PRKAA2 antibody;Protein kinase AMP activated alpha 2 catalytic subunit antibody;Protein kinase AMP activated catalytic subunit alpha 2 antibody

SUBCELLULAR LOCATION

Nucleus, Cytoplasm.

FUNCTION

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.