PRODUCT CODE: ER1706-60

AMACR Rabbit Polyclonal Antibody (ER1706-60)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of AMACR on different lysates using anti-AMACR antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Mouse liver tissue <br />
    Lane 2: SiHa<br />
 Lane 3: SW480<br />
    Lane 4: Human kidney tissue
  • Western blot analysis of AMACR on different lysates using anti-AMACR antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Mouse liver tissue <br />
    Lane 2: SiHa<br />
 Lane 3: SW480<br />
    Lane 4: Human kidney tissue
  • ICC staining AMACR in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-AMACR antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-AMACR antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-AMACR antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of PC-3M cells with AMACR antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of AMACR on different lysates using anti-AMACR antibody at 1/1,000 dilution.
Positive control:
Lane 1: Mouse liver tissue
Lane 2: SiHa
Lane 3: SW480
Lane 4: Human kidney tissue

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

AMACR Rabbit Polyclonal Antibody (ER1706-60)

Immunogen

Recombinant protein within human amacr aa 1-382 / 382.

Host

Rabbit

Positive Control

Human kidney tissue, mouse liver tissue lysate, Siha, SW480, HepG2, human liver tissue, human colon cancer tissue, PC-3M.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

42 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:100

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

AMACR

SYNONYMS

2 arylpropionyl CoA epimerase antibody; 2 methylacyl CoA racemase antibody; 2-methylacyl-CoA racemase antibody; Alpha methylacyl CoA racemase antibody; Alpha methylacyl Coenzyme A racemase antibody; Alpha methylacyl-CoA racemase deficiency, included antibody; Alpha-methylacyl-CoA racemase antibody; Amacr antibody; AMACR deficiency, included antibody; AMACR_HUMAN antibody; CBAS4 antibody; Da1-8 antibody; EC 5.1.99.4 antibody; Macr1 antibody; Methylacyl CoA racemase alpha antibody; RACE antibody; RM antibody

SEQUENCE SIMILARITIES

Belongs to the CoA-transferase III family.

SUBCELLULAR LOCATION

Mitochondrion. Peroxisome.

FUNCTION

P504S, also known as AMACR (α-methylacyl-CoA racemase), 2-methylacyl-CoA racemase or RACE, is an enzyme belonging to the caiB/baiF CoA-transferase family. Localizing to the peroxisome and mitochondrion, P504S plays a role in the metabolism of branched-chain fatty acids and bile acid intermediates. More specifically, P504S catalyzes the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers which can then be degraded by peroxisomal β-oxidation. Mutations in the gene encoding P504S can lead to AMACR deficiency, a disease characterized by increased concentrations of pristanic acid that is associated with adult onset sensory motor neuropathy, and/or CBAS4 (congenital bile acid synthesis defect type 4), a disorder characterized by intrahepatic cholestasis, absence of cholic acid from bile, neonatal jaundice and bile duct deficiency. In addition, P504S is overexpressed in prostate cancer and is believed to be functionally important for prostate cancer cell growth.