PRODUCT CODE: R1308-14

Alpha-tubulin Rabbit Polyclonal Antibody (R1308-14)

Applications

  • WB

  • ICC

REACTIVITY

  • Human

  • Rat

  • Mouse

Western blot analysis on Hela (1) and PC12 (2) cell lysates using anti-α-tubulin rabbit polyclonal antibodies.
  • Western blot analysis on Hela (1) and PC12 (2) cell lysates using anti-α-tubulin rabbit polyclonal antibodies.
  • Immunofluorescent staining of A172 cells using anti-α-tubulin rabbit polyclonal antibody.
  • Immunofluorescent staining of Hela cells using anti-α-tubulin rabbit polyclonal antibody.
Western blot analysis on Hela (1) and PC12 (2) cell lysates using anti-α-tubulin rabbit polyclonal antibodies.

Applications

  • WB

  • ICC

REACTIVITY

  • Human

  • Rat

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Alpha-tubulin Rabbit Polyclonal Antibody (R1308-14)

Immunogen

This antibody is produced by immunizing rabbits with a synthetic peptide (klh-coupled) corresponding to internal alpha-tubulin.

Host

Rabbit

Positive Control

Hela, PC12, A172

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Immunogen affinity purified

MOLECULAR WEIGHT

50 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:200

TARGET

UNIPROT #

PROTEIN NAME

Alpha-tubulin

SYNONYMS

TUBA1 antibody; TUBA1A antibody; TUBA2 antibody; TUBA3 antibody; TUBA3C antibody; TUBA4A antibody; Tubulin, alpha 1a antibody; Tubulin, alpha 3c antibody; Tubulin, alpha 4a antibody

SEQUENCE SIMILARITIES

Belongs to the tubulin family.

POST-TRANSLATIONAL MODIFICATION

Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold.; Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).; Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen. This modification has been correlated with increased microtubule stability, intracellular transport and ciliary assembly.; Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability.

SUBCELLULAR LOCATION

Cytoplasm ,cytoskeleton

FUNCTION

The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.