PRODUCT CODE: ET1706-44

Alpha Fodrin Recombinant Rabbit Monoclonal Antibody [JU32-09] (ET1706-44)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Alpha Fodrin on  different lysates using anti-Alpha Fodrin antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: Hela<br />
  Lane 2: Jurkat<br />
  Lane 3: NIH-3T3<br />
  Lane 4:Rat brain<br />
  Lane 5:Mouse brain
  • Western blot analysis of Alpha Fodrin on  different lysates using anti-Alpha Fodrin antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: Hela<br />
  Lane 2: Jurkat<br />
  Lane 3: NIH-3T3<br />
  Lane 4:Rat brain<br />
  Lane 5:Mouse brain
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Alpha Fodrin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Alpha Fodrin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-Alpha Fodrin antibody. Counter stained with hematoxylin.
Western blot analysis of Alpha Fodrin on different lysates using anti-Alpha Fodrin antibody at 1/500 dilution.
Positive control:
Lane 1: Hela
Lane 2: Jurkat
Lane 3: NIH-3T3
Lane 4:Rat brain
Lane 5:Mouse brain

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Alpha Fodrin Recombinant Rabbit Monoclonal Antibody [JU32-09] (ET1706-44)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, NIH-3T3, Jurkat, rat brain tissue lysate, mouse brain tissue, human liver cancer tissue, human pancreas tissue, mouse cerebellum tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU32-09

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

285/150 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Alpha Fodrin

SYNONYMS

(ALPHA)II-SPECTRIN antibody; Alpha II Spectrin antibody; Alpha-II spectrin antibodybrain antibody; EIEE5 antibody; FLJ17738 antibody; FLJ44613 antibody; Fodrin alpha chain antibody; Fodrin, alpha antibody; NEAS antibody; Non erythrocytic spectrin alpha antibody; non-erythroid alpha chain antibody; SPECA antibody; Spectrin alpha chain antibody; Spectrin alpha chain brain antibody; Spectrin alpha non erythrocytic 1 antibody; Spectrin antibody; Spectrin non erythroid alpha chain antibody; Spectrin, alpha, non-erythrocytic 1 (alpha-fodrin) antibody; Spectrin, nonerythroid, alpha subunit antibody; Spna2 antibody; SPTA 2 antibody; SPTA2 antibody; SPTA2_HUMAN antibody; SPTAN 1 antibody; SPTAN1 antibody

SEQUENCE SIMILARITIES

Belongs to the spectrin family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro.

SUBCELLULAR LOCATION

Cytoskeleton.

FUNCTION

Alpha Fodrin also named Spectrin. Spectrin, an actin binding protein that is a major component of the cytoskeletal superstructure of the erythrocyte plasma membrane, is essential in determining the properties of the membrane including its shape and deformability. Spectrins function as membrane organizers and stabilizers, composed of nonhomologous α and β chains, which aggregate side-to-side in an antiparallel fashion to form dimers, tetramers, and higher polymers. Spectrin α I and spectrin β I are present in erythrocytes, whereas spectrin α II (also designated fodrin α) and spectrin β II (also designated fodrin β) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Activation of calpain results in the breakdown of spectrin α II, a neuronal cytoskeleton protein.