PRODUCT CODE: ER1803-60

alpha Actinin Rabbit Polyclonal Antibody (ER1803-60)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of alpha Actinin on different lysates using anti-alpha Actinin antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: A431<br />
           Lane 2: Rat colon tissue<br />
  Lane 3: Mouse colon tissue
  • Western blot analysis of alpha Actinin on different lysates using anti-alpha Actinin antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: A431<br />
           Lane 2: Rat colon tissue<br />
  Lane 3: Mouse colon tissue
  • ICC staining alpha Actinin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining alpha Actinin in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining alpha Actinin in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-alpha Actinin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-alpha Actinin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-alpha Actinin antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of MG-63 cells with alpha Actinin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of alpha Actinin on different lysates using anti-alpha Actinin antibody at 1/500 dilution.
Positive control:
Lane 1: A431
Lane 2: Rat colon tissue
Lane 3: Mouse colon tissue

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

alpha Actinin Rabbit Polyclonal Antibody (ER1803-60)

Immunogen

Recombinant protein within human alpha actinin aa 350-650.

Host

Rabbit

Positive Control

A431, rat colon tissue lysates, mouse colon tissue lysates, MG-63, SiHa, human colon cancer tissue, human kidney tissue, mouse colon tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

103 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:100

  • IHC-P

  • 1:50 -1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

alpha Actinin

SYNONYMS

actinin 1 smooth muscle antibody; Actinin alpha 1 antibody; actinin, alpha 1 antibody; ACTN 1 antibody; Actn1 antibody; ACTN1_HUMAN antibody; Alpha Actinin 1 antibody; Alpha actinin cytoskeletal isoform antibody; Alpha-actinin cytoskeletal isoform antibody; Alpha-actinin-1 antibody; BDPLT15 antibody; F actin cross linking protein antibody; F-actin cross-linking protein antibody; FLJ40884 antibody; FLJ54432 antibody; Non muscle alpha actinin 1 antibody; Non-muscle alpha-actinin-1 antibody

SEQUENCE SIMILARITIES

Belongs to the alpha-actinin family.

SUBCELLULAR LOCATION

Plasma membrane. Cytoskeleton.

FUNCTION

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.