PRODUCT CODE: ET1706-05

alpha Actinin 4 Recombinant Rabbit Monoclonal Antibody [JU20-23] (ET1706-05)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IP

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of alpha Actinin 4 on different lysates using anti-alpha Actinin 4 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: Hela<br />
      Lane 2: PC-12<br />
 Lane 3: NIH-3T3<br />
   Lane 4: Rat liver tissue<br />
 Lane 5: A431 <br />
     Lane 6: HepG2
  • Western blot analysis of alpha Actinin 4 on different lysates using anti-alpha Actinin 4 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: Hela<br />
      Lane 2: PC-12<br />
 Lane 3: NIH-3T3<br />
   Lane 4: Rat liver tissue<br />
 Lane 5: A431 <br />
     Lane 6: HepG2
  • ICC staining alpha Actinin 4 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining alpha Actinin 4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining alpha Actinin 4 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-alpha Actinin 4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-alpha Actinin 4 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of A549 cells with alpha Actinin 4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of alpha Actinin 4 on different lysates using anti-alpha Actinin 4 antibody at 1/500 dilution.
Positive control:
Lane 1: Hela
Lane 2: PC-12
Lane 3: NIH-3T3
Lane 4: Rat liver tissue
Lane 5: A431
Lane 6: HepG2

Applications

  • WB

  • ICC

  • IF

  • IP

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

alpha Actinin 4 Recombinant Rabbit Monoclonal Antibody [JU20-23] (ET1706-05)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, PC-12, NIH-3T3, rar liver tissue lysate, A431, HepG2, A549, LOVO, human colon cancer tissue, mouse colon tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU20-23

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

105 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

alpha Actinin 4

SYNONYMS

actinin 4 antibody; Actinin alpha 4 antibody; actinin4 antibody; ACTN 4 antibody; ACTN4 antibody; ACTN4_HUMAN antibody; Alpha-actinin-4 antibody; DKFZp686K23158 antibody; F actin cross linking protein antibody; F-actin cross-linking protein antibody; Focal segmental glomerulosclerosis 1 antibody; FSGS 1 antibody; FSGS antibody; FSGS1 antibody; Non muscle alpha actinin 4 antibody; Non-muscle alpha-actinin 4 antibody

SEQUENCE SIMILARITIES

Belongs to the alpha-actinin family.

TISSUE SPECIFICITY

Widely expressed.

SUBCELLULAR LOCATION

Cell junction. Cytoplasm. Nucleus.

FUNCTION

The spectrin gene family encodes a diverse group of cytoskeletal proteins that include spectrins, dystrophins and α-actinins. There are four tissue-specific α-actinins, namely α-actinin-1, α-actinin-2, α-actinin-3 and α-actinin-4, which are localized to muscle and non-muscle cells, including skeletal, cardiac and smooth muscle cells, as well as within the cytoskeleton. Each α-actinin protein contains one Actin-binding domain, two calponin-homology domains, two EF-hand domains and four spectrin repeats, through which they function as bundling proteins that can cross-link F-Actin, thus anchoring Actin to a variety of intracellular structures. Defects in the gene encoding α-actinin-4 are the cause of focal segmental glomerulosclerosis 1 (FSGS1), a common renal lesion characterized by decreasing kidney function and, ultimately, renal failure. are actually sensitive to the Profilin proteins in these foods.