PRODUCT CODE: 0407-3

alpha-Actin (cardiac actin) Rabbit Polyclonal Antibody (0407-3)

  • Zebrafish

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

Western blot analysis on Mouse Heart using anti-a-Actin polyclonal antibody
  • Western blot analysis on Mouse Heart using anti-a-Actin polyclonal antibody
  • Western blot analysis of Alpha-cardiac actin on hybrid fish (crucian-carp) heart tissue lysate using anti-Alpha-cardiac actin antibody at 1/500 dilution.
Western blot analysis on Mouse Heart using anti-a-Actin polyclonal antibody

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

alpha-Actin (cardiac actin) Rabbit Polyclonal Antibody (0407-3)

Immunogen

Synthetic peptide within human alpha-actin (cardiac actin) aa 1-50 / 377.

Host

Rabbit

Positive Control

Mouse heart

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Immunogen affinity purified

MOLECULAR WEIGHT

42 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

TARGET

UNIPROT #

PROTEIN NAME

alpha-Actin (cardiac actin)

SYNONYMS

ACTC antibody; ACTC_HUMAN antibody; ACTC1 antibody; Actin alpha cardiac muscle 1 antibody; Actin antibody; alpha cardiac muscle 1 antibody; Alpha-cardiac actin antibody; ASD5 antibody; CMD1R antibody; CMH11 antibody; LVNC4 antibody

SEQUENCE SIMILARITIES

Belongs to the actin family.

POST-TRANSLATIONAL MODIFICATION

Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promotes actin repolymerization.; Monomethylation at Lys-86 (K86me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.; Methylated at His-75 by SETD3.; (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding. The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners.

SUBCELLULAR LOCATION

Cytoplasma , etc.

FUNCTION

Actins are highly conserved proteins expressed in all eucaryotic cells. Actin filaments form part of the cytoskeleton and play essential roles in regulating cell shape and movement. Six distinct actin isotypes have been identified in mammalian cells. Each is encoded by a separated gene and is expressed in a developmentally regulated and tissue-specific manner, alpha and beta cytoplasmic actins are expressed in a wide variety of cells; whereas, expression of alpha skeletal, alpha cardiac, alpha vascular, and gamma enteric actins are more restricted to specialized muscle cell type. Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Further more, expression of smooth muscle alpha actin is regulated by hormones, cell proliferation , and altered by pathological conditions including oncogenic transformation and atherosclerosis