PRODUCT CODE: ER1802-27

Alpha-1 acid glycoprotein Rabbit Polyclonal Antibody (ER1802-27)

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Alpha-1 Acid Glycoprotein on human liver tissue lysates using anti-Alpha-1 acid glycoprotein antibody at 1/500 dilution.
  • Western blot analysis of Alpha-1 Acid Glycoprotein on human liver tissue lysates using anti-Alpha-1 acid glycoprotein antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Alpha-1 Acid Glycoprotein antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Alpha-1 Acid Glycoprotein antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Alpha-1 Acid Glycoprotein antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with Alpha-1 Acid Glycoprotein antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Alpha-1 Acid Glycoprotein on human liver tissue lysates using anti-Alpha-1 acid glycoprotein antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Alpha-1 acid glycoprotein Rabbit Polyclonal Antibody (ER1802-27)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human liver tissue lysates, rat liver tissue, human liver tissue, mouse liver tissue, HepG2.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified

MOLECULAR WEIGHT

35 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Alpha-1 acid glycoprotein

SYNONYMS

A1AG1_HUMAN antibody; AGP 1 antibody; AGP A antibody; AGP antibody; AGP1 antibody; Alpha 1 acid glycoprotein antibody; Alpha-1-acid glycoprotein 1 antibody; alpha-1-AGP antibody; Epididymis secretory sperm binding protein Li 153w antibody; glycoprotein, alpha-1-acid, of serum antibody; HEL S 153w antibody; OMD 1 antibody; ORM antibody; ORM1 antibody; Orosomucoid 1 antibody; Orosomucoid-1 antibody

SEQUENCE SIMILARITIES

Belongs to the calycin superfamily. Lipocalin family.

TISSUE SPECIFICITY

Expressed by the liver and secreted in plasma.

POST-TRANSLATIONAL MODIFICATION

N-glycosylated. N-glycan heterogeneity at Asn-33: Hex5HexNAc4 (minor), Hex6HexNAc5 (major) and dHex1Hex6HexNAc5 (minor).

SUBCELLULAR LOCATION

Secreted.

FUNCTION

AGP (α1-acid glycoprotein) is an acute phase plasma protein synthesized by the liver. It functions to regulate the interaction between blood cells and endothelial cells, and together with haptoglobin and C reactive protein, it also mediates the extravasation of cells during infection and inflammation. Expression of AGP is induced by acute-phase stimulatory agents such as bacterial lipopolysaccharides. AGP has a high affinity, low capacity binding for basic drugs at physiological pH. In human plasma, AGP is found at levels of 0.5-1.4 mg/ml, though this is elevated during acute inflammation, and, as a result, levels of this protein can be used to diagnose inflammatory conditions. AGP-1 and AGP-2 contain five and six potential N-glycosylation sites, respectively. Abnormal expression of the APG-1 gene is linked to sarcoidosis and other immunogenetic diseases, while mutations in the APG-2 gene are associated with different types of carcinomas.