Alkaline Phosphatase Recombinant Rabbit Monoclonal Antibody [SA40-00] (ET1601-21)
Catalog# ET1601-21
Rabbit Monoclonal to Alkaline Phosphatase
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WB
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IHC-P
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IF-Tissue
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Human
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Mouse
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Rat
Lane 1: Saos-2 cell lysate (15 µg/Lane)
Lane 2: HeLa cell lysate (15 µg/Lane)
Lane 3: A549 cell lysate (15 µg/Lane)
Lane 4: Mouse liver tissue lysate (20 µg/Lane)
Lane 5: Rat liver tissue lysate (20 µg/Lane)
Predicted band size: 57 kDa
Observed band size: 75 kDa
Exposure time: 24 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-21) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Safety datasheet
Select your chosen country/region
- MSDS_HUABIO.pdf
- MSDS_HUABIO.pdf
- MSDS_ET1601-21_Europe.pdf
- No MSDS Found
COA DOWNLOAD
- No COA Found
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Alkaline Phosphatase Recombinant Rabbit Monoclonal Antibody [SA40-00] (ET1601-21)
Immunogen
Synthetic peptide within human alkaline phosphatase aa 18-50.
Host
Rabbit
Positive Control
Saos-2 cell lysate, HeLa cell lysate, A549 cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate, HepG2, SW480, mouse kidney tissue, mouse jawbone tissue, rat liver tissue, mouse liver tissue, Hela.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SA40-00
RRID
APPLICATION DILUTION
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WB
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1:5,000
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IHC-P
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1:1,000-1:8,000
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IF-Tissue
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1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 57 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Alkaline Phosphatase
SYNONYMS
Alkaline phosphatase antibody;Alkaline phosphatase placental antibody;Alkaline phosphatase placental type antibody;Alkaline phosphatase Regan isozyme antibody;ALP antibody;Alp1 antibody;ALPP antibody;FLJ61142 antibody;Germ-cell alkaline phosphatase antibody;nagao isozyme antibody;OTTHUMP00000164354 antibody;PALP antibody;Placental alkaline phosphatase 1 antibody;placental heat-stable alkaline phosphatase antibody;placental type antibody;PLAP antibody;PLAP-1 antibody;PLAP1 antibody;PPB1_HUMAN antibody
SEQUENCE SIMILARITIES
Belongs to the alkaline phosphatase family.
POST-TRANSLATIONAL MODIFICATION
N-glycosylated.
SUBCELLULAR LOCATION
Cell membrane, Mitochondrion membrane, Mitochondrion intermembrane space, Extracellular vesicle membrane.
FUNCTION
This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects.
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