PRODUCT CODE: ET1705-74

ALIX Recombinant Rabbit Monoclonal Antibody [JM85-31] (ET1705-74)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of ALIX on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-74, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Hela cell lysate<br />
Lane 2: SH-SY5Y cell lysate
  • Western blot analysis of ALIX on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-74, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Hela cell lysate<br />
Lane 2: SH-SY5Y cell lysate
  • Immunohistochemical analysis of paraffin-embedded rat stomach tissue using anti-ALIX antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-74, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-ALIX antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-74, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-ALIX antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-74, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of ALIX was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1705-74, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of ALIX on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-74, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: SH-SY5Y cell lysate

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

ALIX Recombinant Rabbit Monoclonal Antibody [JM85-31] (ET1705-74)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela cell lysate, SH-SY5Y cell lysate, rat stomach tissue, human colon carcinoma tissue, human prostate tissue, Jurkat.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM85-31

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

96 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

ALIX

SYNONYMS

AIP1 antibody; ALG 2 interacting protein 1 antibody; ALG-2-interacting protein 1 antibody; ALG2 interacting protein X antibody; Alix antibody; Apoptosis linked gene 2 interacting protein X antibody; Dopamine receptor interacting protein 4 antibody; DRIP4 antibody; Hp95 antibody; KIAA1375 antibody; MGC17003 antibody; PDC6I_HUMAN antibody; PDCD6 interacting protein antibody; PDCD6-interacting protein antibody; PDCD6IP antibody; Programmed cell death 6 interacting protein antibody; Programmed cell death 6-interacting protein antibody

POST-TRANSLATIONAL MODIFICATION

May be phosphorylated on tyrosine residues by activated PDGFRB.

SUBCELLULAR LOCATION

Centrosome, extracellular exosome, cytosol, melanosome, tight junction, midbody ring.

FUNCTION

ALG-2-interacting protein (Alix), also designated programmed cell death 6-interacting protein (PDCD6-interacting protein), is a cytoplasmic protein. Alix interacts with apoptosis-associated proteins (ALG-2 and PDCD6) and with the endocytosis-regulator CIN85. Additionally, Alix interacts with the endosomal sorting complexes required for transport (ESCRT) proteins (Tsg101 and CHMP4) and can associate with HIV-1. The endophilins (SH3P4, SH3P8 and SH3P13), enzymes that change curvature of the membrane that are required for early and late steps of coated vesicle formation, also bind to Alix. Alix is involved in the concentration and sorting of cargo proteins of the multivesicular body for incorporation into vesicles.