ALDH1A1 Recombinant Rabbit Monoclonal Antibody [SY11-02] (ET1605-24)
Catalog# ET1605-24
Rabbit Monoclonal to ALDH1A1
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WB
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IHC-P
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IHC-Fr
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IP
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Human
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Mouse
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Rat
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Cynomolgus monkey
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Pig
Western blot analysis of ALDH1A1 on different lysates with Rabbit anti-ALDH1A1 antibody (ET1605-24) at 1/2,000 dilution.
Lane 1: A549-WT cell lysate
Lane 2: A549-KD ALDH1A1 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 55 kDa
Observed band size: 50 kDa
Exposure time: 5 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-24) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
ALDH1A1 Recombinant Rabbit Monoclonal Antibody [SY11-02] (ET1605-24)
Immunogen
Synthetic peptide within human aldh1a1 aa 300-360.
Host
Rabbit
Positive Control
A549 cell lysate, mouse lung tissue lysate, human liver tissue lysate, human kidney tissue lysate, human liver tissue, human kidney tissue, mouse striatum tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SY11-02
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:2,000
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IHC-P
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1:100-1:200
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IHC-Fr
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1:200
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IP
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Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 55 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
ALDH1A1
SYNONYMS
Acetaldehyde dehydrogenase 1 antibody;AHD2 antibody;AL1A1_HUMAN antibody;ALDC antibody;Aldehyde dehydrogenase 1 family member A1 antibody;Aldehyde dehydrogenase 1 soluble antibody;Aldehyde dehydrogenase 1A1 antibody;Aldehyde dehydrogenase antibody;Aldehyde dehydrogenase cytosolic antibody;Aldehyde dehydrogenase family 1 member A1 antibody;Aldehyde dehydrogenase liver cytosolic antibody;ALDH 1 antibody;ALDH 1A1 antibody;ALDH class 1 antibody;ALDH, liver cytosolic antibody;ALDH-E1 antibody;ALDH1 A1 antibody;ALDH1 antibody;ALDH11 antibody;ALDH1A1 antibody;ALHDII antibody;cytosolic antibody;epididymis luminal protein 12 antibody;epididymis luminal protein 9 antibody;epididymis secretory sperm binding protein Li 53e antibody;HEL-S-53e antibody;MGC2318 antibody;PUMB1 antibody;RALDH 1 antibody;RalDH1 antibody;Retinal dehydrogenase 1 antibody
SEQUENCE SIMILARITIES
Belongs to the aldehyde dehydrogenase family.
POST-TRANSLATIONAL MODIFICATION
The N-terminus is blocked most probably by acetylation.
SUBCELLULAR LOCATION
Cytoplasm,Cell projection.
FUNCTION
Aldehyde dehydrogenases (ALDHs) mediate NADP+-dependent oxidation of aldehydes into acids during the detoxification of alcohol-derived acetaldehyde; metabolism of corticosteroids, biogenic amines and neurotransmitters; and lipid peroxidation. ALDH1A1, also designated retinal dehydrogenase 1 (RalDH1 or RALDH1), aldehyde dehydrogenase family 1 member A1, aldehyde dehydrogenase cytosolic, ALDHII, ALDH-E1 or ALDH E1, is a retinal dehydrogenase that participates in the biosynthesis of retinoic acid (RA). There are two major liver isoforms of ALDH1 that can localize to cytosolic or mitochondrial space. The ALDH1A2 (RALDH2, RALDH2-T) gene produces three different transcripts and also catalyzes the synthesis of RA from retinaldehyde. ALDH1A3 (ALDH6, RALDH3, ALDH1A6) is a 37 kb gene that consists of 13 exons and produces a major transcript of approximately 3.5 kb most abundant in salivary gland, stomach and kidney. ALDH3A1 (stomach type, ALDH3, ALDHIII) forms a cytoplasmic homodimer that preferentially oxidizes aromatic aldehyde substrates. ALDH genes upregulate as a part of the oxidative stress response, and appear to be abundant in certain tumors that have an accelerated metabolism toward chemotherapy agents.
CITATIONS
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Photodynamic therapy of lung cancer with photosensitizers based on polycationic derivatives of synthetic bacteriochlorin (experimental study)
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IHC
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Human
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Mouse
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Citation
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Qiu Ruiqi,et al
AAV2-PDE6B restores retinal structure and function in the retinal degeneration 10 mouse model of retinitis pigmentosa by promoting phototransduction and inhibiting apoptosis
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WB
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Mouse
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Citation
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Qiu Ruiqi,et al
AAV2-PDE6B restores retinal structure and function in the retinal degeneration 10 mouse model of retinitis pigmentosa by promoting phototransduction and inhibiting apoptosis
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WB
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Mouse
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Citation
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