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PRODUCT CODE: ET7108-64

Alcohol Dehydrogenase Recombinant Rabbit Monoclonal Antibody [JG82-31] (ET7108-64)

  • Recombinant

Applications

  • WB

  • IHC-P

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of Alcohol Dehydrogenase on rat liver tissue lysate using anti-Alcohol Dehydrogenase antibody at 1/1,000 dilution.
  • Western blot analysis of Alcohol Dehydrogenase on rat liver tissue lysate using anti-Alcohol Dehydrogenase antibody at 1/1,000 dilution.
  • ICC staining Alcohol Dehydrogenase in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Alcohol Dehydrogenase antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Alcohol Dehydrogenase antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Alcohol Dehydrogenase antibody. Counter stained with hematoxylin.
Western blot analysis of Alcohol Dehydrogenase on rat liver tissue lysate using anti-Alcohol Dehydrogenase antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Alcohol Dehydrogenase Recombinant Rabbit Monoclonal Antibody [JG82-31] (ET7108-64)

Immunogen

Recombinant protein within human alcohol dehydrogenase aa 1-200.

Host

Rabbit

Positive Control

Rat liver tissue, human liver tissue, mouse liver tissue, HepG2.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JG82-31

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

40 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:100

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

P07327

PROTEIN NAME

Alcohol Dehydrogenase

SYNONYMS

ADH alpha subunit antibody; ADH antibody; ADH1 antibody; ADH1A antibody; ADH1A_HUMAN antibody; Alcohol dehydrogenase 1 (class I), alpha polypeptide antibody; Alcohol dehydrogenase 1 antibody; Alcohol dehydrogenase 1A (class I), alpha polypeptide antibody; Alcohol dehydrogenase 1A antibody; Alcohol dehydrogenase subunit alpha antibody; Aldehyde reductase antibody

SEQUENCE SIMILARITIES

Belongs to the zinc-containing alcohol dehydrogenase family.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

The alcohol dehydrogenase family of proteins metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class I alcohol dehydrogenase, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha (ADH1A), beta (ADH1B) and gamma (ADH1C) subunits are tandemly organized on chromosome 4q22 as a gene cluster. The alpha form of ADH is monomorphic and predominant in fetal and infant livers, becoming less active in gestation and only weakly active during adulthood. The genes encoding beta and gamma subunits, however, are polymorphic and strongly expressed in adult livers. With the coenzyme NAD, ADH catalyzes the reversible conversion of organic alcohols to ketones or aldehydes. The physiologic function for ADH in the liver is the removal of ethanol formed by microorganisms in the intestinal tract.