PRODUCT CODE: EM1901-81

Albumin Mouse Monoclonal Antibody [1-G10] (EM1901-81)

Applications

  • WB

  • FC

  • ELISA

REACTIVITY

  • Human

Western blot analysis of Albumin on human plasma tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-81, 1/50000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of Albumin on human plasma tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-81, 1/50000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Flow cytometric analysis of Albumin was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-81, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with Alexa FluorTM488 Goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; blcak).
Western blot analysis of Albumin on human plasma tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-81, 1/50000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • FC

  • ELISA

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Albumin Mouse Monoclonal Antibody [1-G10] (EM1901-81)

Immunogen

Native protein.

Host

Mouse

Positive Control

Human plasma tissue lysates, HepG2.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

1-G10

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

69 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:10,000-1:50,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Albumin

SYNONYMS

alb antibody; ALBU_HUMAN antibody; Albumin (32 AA) antibody; Albumin (AA 34) antibody; Albumin antibody; Analbuminemia antibody; Bisalbuminemia antibody; Cell growth inhibiting protein 42 antibody; DKFZp779N1935 antibody; Dysalbuminemic hyperthyroxinemia antibody; Growth inhibiting protein 20 antibody; HSA antibody; Hyperthyroxinemia dysalbuminemic antibody; PRO0883 antibody; PRO0903 antibody; PRO1341 antibody; Serum albumin antibody

SEQUENCE SIMILARITIES

Belongs to the ALB/AFP/VDB family.

TISSUE SPECIFICITY

Plasma.

POST-TRANSLATIONAL MODIFICATION

Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.; Glycated in diabetic patients.; Phosphorylated by FAM20C in the extracellular medium.; Acetylated on Lys-223 by acetylsalicylic acid.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs (Probable). Its main function is the regulation of the colloidal osmotic pressure of blood (Probable). Major zinc transporter in plasma, typically binds about 80% of all plasma zinc . Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli . Does not prevent iron uptake by the bacterial siderophore aerobactin .