Lane 1: JAR
Lane 2: HUVEC
Lane 3: Hela
Recombinant Rabbit monoclonal primary
Alas1 Recombinant Rabbit Monoclonal Antibody [SA70-04] (ET1601-26)
Recombinant protein within human alas1 aa 510-640 / 640.
HUVEC, MCF-7, Hela, HepG2, JAR.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A purified.
5 aminolevulinate synthase antibody; 5 aminolevulinate synthase nonspecific mitochondrial antibody; 5 aminolevulinic acid synthase antibody; 5-aminolevulinate synthase antibody; 5-aminolevulinic acid synthase 1 antibody; Alas 1 antibody; ALAS 3 antibody; ALAS antibody; ALAS H antibody; ALAS HOUSEKEEPING TYPE antibody; ALAS N antibody; ALAS-H antibody; alaS1 antibody; ALAS3 antibody; ALASH antibody; Aminolevulinate delta synthase 1 antibody; Aminolevulinic acid synthase 1 antibody; Delta ALA synthetase antibody; Delta aminolevulinate synthase antibody; Delta-ALA synthase 1 antibody; Delta-aminolevulinate synthase 1 antibody; HEM1_HUMAN antibody; MIG 4 antibody; MIG4 antibody; Migration inducing protein 4 antibody; mitochondrial antibody; nonspecific antibody
Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.
5-aminolevulinate synthase 1 (ALAS-H) and 2 (ALAS-E) are two isoforms of ALAS, an enzyme catalyzing the first step of the heme biosynthetic pathway in mammals. The erythroid-specific isoenzyme, ALAS-E, regulates the first step of hematopoietic cell differentation and iron metabolism in the liver. ALAS-H is a housekeeping protein which mediates synthesis of early heme in the mitochondria of most cells. Succinyl CoA associates with ALAS-E in protein conformation change and translocation of ALAS-E into the mitochondria and does not interact with ALAS-H. The ALAS-E 5'-flanking region contains binding sites for nuclear activators such as GATA-1, NF-E2 and EKLF. Since the ALAS gene maps to the X chromosome, mutation of the gene leads to the pyridoxine-refractory X-linked sideroblastic anemia.