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PRODUCT CODE: ET1601-26

Alas1 Recombinant Rabbit Monoclonal Antibody [SA70-04] (ET1601-26)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

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Western blot analysis of Alas1 on different cell lysates using anti-Alas1 antibody at 1/1,000 dilution.<br /> Positive control: <br /> Lane 1: JAR <br /> Lane 2: HUVEC <br /> Lane 3: Hela
  • Western blot analysis of Alas1 on different cell lysates using anti-Alas1 antibody at 1/1,000 dilution.<br /> Positive control: <br /> Lane 1: JAR <br /> Lane 2: HUVEC <br /> Lane 3: Hela
  • ICC staining Alas1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Alas1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Alas1 in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of Alas1 on different cell lysates using anti-Alas1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: JAR
Lane 2: HUVEC
Lane 3: Hela

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Alas1 Recombinant Rabbit Monoclonal Antibody [SA70-04] (ET1601-26)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

HUVEC, MCF-7, Hela, HepG2, JAR.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SA70-04

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

71 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:50-1:200

TARGET

UNIPROT #

P13196

PROTEIN NAME

Alas1

SYNONYMS

5 aminolevulinate synthase antibody; 5 aminolevulinate synthase nonspecific mitochondrial antibody; 5 aminolevulinic acid synthase antibody; 5-aminolevulinate synthase antibody; 5-aminolevulinic acid synthase 1 antibody; Alas 1 antibody; ALAS 3 antibody; ALAS antibody; ALAS H antibody; ALAS HOUSEKEEPING TYPE antibody; ALAS N antibody; ALAS-H antibody; alaS1 antibody; ALAS3 antibody; ALASH antibody; Aminolevulinate delta synthase 1 antibody; Aminolevulinic acid synthase 1 antibody; Delta ALA synthetase antibody; Delta aminolevulinate synthase antibody; Delta-ALA synthase 1 antibody; Delta-aminolevulinate synthase 1 antibody; HEM1_HUMAN antibody; MIG 4 antibody; MIG4 antibody; Migration inducing protein 4 antibody; mitochondrial antibody; nonspecific antibody

SEQUENCE SIMILARITIES

Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.

SUBCELLULAR LOCATION

Mitochondrion matrix

FUNCTION

5-aminolevulinate synthase 1 (ALAS-H) and 2 (ALAS-E) are two isoforms of ALAS, an enzyme catalyzing the first step of the heme biosynthetic pathway in mammals. The erythroid-specific isoenzyme, ALAS-E, regulates the first step of hematopoietic cell differentation and iron metabolism in the liver. ALAS-H is a housekeeping protein which mediates synthesis of early heme in the mitochondria of most cells. Succinyl CoA associates with ALAS-E in protein conformation change and translocation of ALAS-E into the mitochondria and does not interact with ALAS-H. The ALAS-E 5'-flanking region contains binding sites for nuclear activators such as GATA-1, NF-E2 and EKLF. Since the ALAS gene maps to the X chromosome, mutation of the gene leads to the pyridoxine-refractory X-linked sideroblastic anemia.