Alas1 Recombinant Rabbit Monoclonal Antibody [SA70-04] (ET1601-26)
Catalog# ET1601-26
Rabbit Monoclonal to Alas1
Tested for
-
WB
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
Western blot analysis of Alas1 on different lysates with Rabbit anti-Alas1 antibody (ET1601-26) at 1/1,000 dilution.
Lane 1: A549-WT cell lysate
Lane 2: A549-KD Alas1 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 71 kDa
Observed band size: 65 kDa
Exposure time: 40 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-26) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A549-WT cell lysate
Lane 2: A549-KD Alas1 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 71 kDa
Observed band size: 65 kDa
Exposure time: 40 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-26) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.