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PRODUCT CODE: ET1610-75

AKAP14 Recombinant Rabbit Monoclonal Antibody [SC0667] (ET1610-75)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of AKAP14 on different lysates using anti-AKAP14 antibody at 1/1,000 dilution.<br /> Positive control: <br /> Lane 1: Jurkat <br /> Lane 2: A549
  • Western blot analysis of AKAP14 on different lysates using anti-AKAP14 antibody at 1/1,000 dilution.<br /> Positive control: <br /> Lane 1: Jurkat <br /> Lane 2: A549
  • ICC staining AKAP14 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining AKAP14 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining AKAP14 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of AKAP14 on different lysates using anti-AKAP14 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Jurkat
Lane 2: A549

Applications

  • WB

  • ICC

  • IF

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

AKAP14 Recombinant Rabbit Monoclonal Antibody [SC0667] (ET1610-75)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

HUVEC, Jurkat, Hela, A549.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SC0667

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

23 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:50-1:200

TARGET

UNIPROT #

Q86UN6

PROTEIN NAME

AKAP14

SYNONYMS

A kinase (PRKA) anchor protein 14 antibody; A kinase anchor protein 14 antibody; A kinase anchor protein 28 kDa antibody; A-kinase anchoring protein 28 antibody; AKAP14 antibody; AKAP28 antibody; PRKA14 antibody; Protein kinase A anchoring protein 14 antibody

TISSUE SPECIFICITY

Present in cilia (at protein level). Expressed in tissues containing axoneme-based organelles (cilia and/or flagella): trachea and testis. Highly expressed in airway cilia.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The protein anchors PKA in ciliary axonemes and, in this way, may play a role in regulating ciliary beat frequency. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. Binds to type II regulatory subunits of protein kinase A and anchors/targets them.