AKAP14 Recombinant Rabbit Monoclonal Antibody [SC0667] (ET1610-75)
Catalog# ET1610-75
Rabbit Monoclonal to AKAP14
Tested for
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WB
Reacts with
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Human
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Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of AKAP14 on different lysates with Rabbit anti-AKAP14 antibody (ET1610-75) at 1/5,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: HUVEC cell lysate
Lane 3: A549 cell lysate
Lane 4: DU 145 cell lysate
Lane 5: Mouse testis tissue lysate
Lane 6: Rat testis tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-75) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Jurkat cell lysate
Lane 2: HUVEC cell lysate
Lane 3: A549 cell lysate
Lane 4: DU 145 cell lysate
Lane 5: Mouse testis tissue lysate
Lane 6: Rat testis tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-75) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.