PRODUCT CODE: M1509-2

AK6 Mouse Monoclonal Antibody [B1-F4] (M1509-2)

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of AK6 on MCF-7 cell (1) and human lung tissue (2) lysate using anti-AK6 antibody at 1/1000 dilution.
  • Western blot analysis of AK6 on MCF-7 cell (1) and human lung tissue (2) lysate using anti-AK6 antibody at 1/1000 dilution.
  • ICC staining AK6 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Flow cytometric analysis of MCF-7 cells with AK6 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of AK6 on MCF-7 cell (1) and human lung tissue (2) lysate using anti-AK6 antibody at 1/1000 dilution.

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

AK6 Mouse Monoclonal Antibody [B1-F4] (M1509-2)

Immunogen

Peptide

Host

Mouse

Positive Control

Human lung tissue lysate, MCF-7. 

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

B1-F4

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

20 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:2,000-1:5,000

  • ICC

  • 1:50-1:200

  • FC

  • 1:50-1:00

TARGET

UNIPROT #

PROTEIN NAME

AK6

SYNONYMS

AD-004 antibody; Adenylate kinase isoenzyme 6 antibody; Adrenal gland protein AD-004 antibody; AK/ATPase antibody; AK6 antibody; CGI-137 antibody; CIP antibody; Coilin-interacting nuclear ATPase protein antibody; Dual activity adenylate kinase/ATPase antibody; hCINAP antibody; KAD6_HUMAN antibody; TAF9 antibody

SEQUENCE SIMILARITIES

Belongs to the adenylate kinase family. AK6 subfamily.

TISSUE SPECIFICITY

Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, chorionic villi and the central nervous system.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

In eukaryotic systems, the process of initiating transcription from protein-coding genes requires the presence of RNA polymerase II and a broad family of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that mediate the transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH, and sequence-specific factors that regulate gene expression. TFIID, one of the basal transcription factors, facilitates the preinitiation complex assembly through direct interactions with the TATA promoter element. TAF9 (transcription initiation factor TFIID subunit 9), also known as TAF9A or TAF II p32, is a 264 amino acid nuclear protein that, is a component of the TFIID complex and is required for cell viability.