AIM2 Rabbit Polyclonal Antibody (HA500270)
Catalog# HA500270
Rabbit Polyclonal to AIM2
-
WB
-
IF-Cell
-
FC
-
Human
Predicted band size: 39 kDa
Observed band size: 39 kDa
Specifications
Product Type
Rabbit polyclonal primary
Product Name
AIM2 Rabbit Polyclonal Antibody (HA500270)
Immunogen
Synthetic peptide within human aim2 aa 50-150 / 343.
Host
Rabbit
Positive Control
Hela cell lysates, A431, Hela.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
-
WB
-
1:500
-
IF-Cell
-
1:50
-
FC
-
1:500-1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 39 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
AIM2
SYNONYMS
Absent in melanoma 2 antibody;AIM 2 antibody;Aim2 antibody;AIM2_HUMAN antibody;Interferon-inducible protein AIM2 antibody;OTTHUMP00000035296 antibody;PYHIN4 antibody
SUBCELLULAR LOCATION
Cytoplasm, Nucleus.
FUNCTION
Involved in innate immune response by recognizing cytosolic double-stranded DNA and inducing caspase-1-activating inflammasome formation in macrophages. Upon binding to DNA is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Detects cytosolic dsDNA of viral and bacterial origin in a non-sequence-specific manner. Can also trigger PYCARD-dependent, caspase-1-independent cell death that involves caspase-8. Tumor suppressor which may act by repressing NF-kappa-B transcriptional activity. Defects in AIM2 may be a cause of microsatellite unstable colon cancers. In absence of dsDNA pyrin and HIN-20 domain can interact inducing a closed conformation; an autoinhibitory mechanism is proposed in which binding to dsDNA liberates the pyrin domain for homotypic downstream signaling interactions with PYCARD.
CITATIONS
-
Hyperglycemia accelerates inflammaging in the gingival epithelium through inflammasomes activation
-
WB
-
IHC
-
Mouse
-
Citation
-
