PRODUCT CODE: ET1704-57

Aggrecan Recombinant Rabbit Monoclonal Antibody [JA40-23] (ET1704-57)

  • Recombinant

Applications

  • IHC-P

REACTIVITY

  • Human

  • Rat

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Immunohistochemical analysis of paraffin-embedded rat cartilage tissue using anti-Aggrecan antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-57, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded rat cartilage tissue using anti-Aggrecan antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-57, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Aggrecan antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-57, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded rat cartilage tissue using anti-Aggrecan antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-57, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications

  • IHC-P

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Aggrecan Recombinant Rabbit Monoclonal Antibody [JA40-23] (ET1704-57)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Rat cartilage tissue, human liver tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA40-23

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

250/246/239 kDa

Isotype

IgG

APPLICATION DILUTION

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Aggrecan

SYNONYMS

ACAN antibody; AGC 1 antibody; AGC1 antibody; AGCAN antibody; Aggrecan 1 (chondroitin sulfate proteoglycan 1, large aggregating proteoglycan, antigen identified by monoclonal antibody; A0122) antibody; Aggrecan 1 antibody; Aggrecan core protein antibody; Aggrecan proteoglycan antibody; Aggrecan structural proteoglycan of cartilage antibody; Aggrecan1 antibody; ATEGQV antibody; Cartilage specific proteoglycan core protein antibody; Chondroitin sulfate proteoglycan 1 antibody; Chondroitin sulfate proteoglycan 1 large aggregating proteoglycan antigen identified by monoclonal antibody; A0122 antibody; Chondroitin sulfate proteoglycan core protein 1 antibody; CSPG 1 antibody; CSPG1 antibody; CSPGCP antibody; JSCATE antibody; Large aggregating proteoglycan antibody; mcspg antibody; mgsk16 antibody; MSK 16 antibody; MSK16 antibody; SEDK antibody

SEQUENCE SIMILARITIES

Belongs to the aggrecan/versican proteoglycan family.

TISSUE SPECIFICITY

Restricted to cartilages.

DEVELOPMENTAL STAGE

Expression was detected in chondrocytes throughout the developing skeleton.

POST-TRANSLATIONAL MODIFICATION

Contains mostly chondroitin sulfate, but also keratan sulfate chains, N-linked and O-linked oligosaccharides. The release of aggrecan fragments from articular cartilage into the synovial fluid at all stages of human osteoarthritis is the result of cleavage by aggrecanase.

SUBCELLULAR LOCATION

Extracellular matrix.

FUNCTION

The large chondroitin sulfate proteoglycan, aggrecan, is the predominant proteoglycan present in cartilage. Aggrecan is a member of the chondroitin sulphate proteoglycan family, which also includes versican/PG-M, neurocan and brevican. Aggrecan is a complex multidomain macromolecule that undergoes extensive processing and post-translational modification. Aggrecan in cartilage forms aggregates with hyaluronan and link protein, embedded in a collagen network. Aggrecan accounts for the compressive stiffness and resilience of the hyaline cartilage. Many forms of inflammatory arthritis are shown to be accompanied with aggrecan degradation and loss from the cartilage. Brevican is a brain proteoglycan of the aggrecan/versican/neurocan family. In the adult brain, the brevican core protein undergoes proteolytic cleavage and exists as a full-length form a carboxy-terminal fragment and an amino-terminal fragment.