PRODUCT CODE: EM1701-31

AFP Mouse Monoclonal Antibody [A5-G3] (EM1701-31)

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

Western blot analysis of AFP on human placenta tissue lysate using anti-AFP antibody at 1/1,000 dilution.
  • Western blot analysis of AFP on human placenta tissue lysate using anti-AFP antibody at 1/1,000 dilution.
  • Western blot analysis of AFP on HepG2 cell lysate using anti-AFP antibody at 1/1,000 dilution.
  • ICC staining AFP (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining AFP (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining AFP (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil cancer tissue using anti-AFP antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-AFP antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-AFP antibody. Counter stained with hematoxylin.
Western blot analysis of AFP on human placenta tissue lysate using anti-AFP antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

AFP Mouse Monoclonal Antibody [A5-G3] (EM1701-31)

Immunogen

Recombinant protein

Host

Mouse

Positive Control

Human placenta tissue lysate, HepG2, Hela, MCF-7, human lung cancer tissue, human liver cancer tissue, human tonsil tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A5-G3

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

68 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

AFP

SYNONYMS

Afp antibody; AFPD antibody; Alpha fetoglobulin antibody; Alpha fetoprotein antibody; Alpha fetoprotein precursor antibody; Alpha-1-fetoprotein antibody; Alpha-fetoglobulin antibody; Alpha-fetoprotein antibody; alpha-fetoprotein, Hereditary persistence of, included antibody; FETA antibody; FETA_HUMAN antibody; Hereditary persistence of alpha fetoprotein antibody; HPAFP antibody

SEQUENCE SIMILARITIES

Belongs to the ALB/AFP/VDB family.

TISSUE SPECIFICITY

Plasma. Synthesized by the fetal liver and yolk sac.

DEVELOPMENTAL STAGE

Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.

POST-TRANSLATIONAL MODIFICATION

Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.; Sulfated.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

α-fetoprotein (AFP) is expressed in fetal liver at varying levels throughout development and is present only in trace amounts in normal adult tissues. AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma. High AFP concentrations have been correlated with tumor cell growth, indicating that AFP can serve as a tumor marker. AFP binds copper, nickel and fatty acids, and in some cases may bind serum albumin or estrogen. It has been demonstrated that the AFP promoter is a target for NF-1 (nuclear factor-1), HNF-1 (hepatocyte nuclear factor-1) and C/EBP transcription factors. While HNF-1 binding to the AFP promoter results in AFP expression, NF-1 binding results in a decrease in AFP promoter activity.

CITATIONS

  • Qin, Jianmin et al.

    Antitumor effects of brucine immuno-nanoparticles on hepatocellular carcinoma in vivo. | Oncology Letters [2018]