AFF4 Rabbit Polyclonal Antibody (HA500590)
Catalog# HA500590
Rabbit Polyclonal to AFF4
Tested for
-
WB
-
IF-Cell
-
FC
Reacts with
-
Human
-
Mouse
-
Rat
Western blot analysis of AFF4 on different lysates with Rabbit anti-AFF4 antibody (HA500590) at 1/5,000 dilution.
Lane 1: MCF7 cell lysate
Lane 2: HeLa cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: SP2/0 cell lysate
Lane 5: Rat pancreas tissue lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 127 kDa
Observed band size: 150 kDa
Exposure time: Lane 1-4: 1 minute 12 seconds; Lane 5: 9 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500590) at 1/5,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: MCF7 cell lysate
Lane 2: HeLa cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: SP2/0 cell lysate
Lane 5: Rat pancreas tissue lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 127 kDa
Observed band size: 150 kDa
Exposure time: Lane 1-4: 1 minute 12 seconds; Lane 5: 9 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500590) at 1/5,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.