ADX Recombinant Rabbit Monoclonal Antibody [JE63-56] (HA721329)
Catalog# HA721329
Rabbit Monoclonal to ADX
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WB
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IHC-P
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IF-Cell
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Human
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Mouse
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Rat
Lane 1: SK-Br-3 cell lysate (15 µg/Lane)
Lane 2: K-562 cell lysate (15 µg/Lane)
Lane 3: HepG2 cell lysate (15 µg/Lane)
Lane 4: 293T cell lysate (15 µg/Lane)
Predicted band size: 19 kDa
Observed band size: 13 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721329) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
ADX Recombinant Rabbit Monoclonal Antibody [JE63-56] (HA721329)
Immunogen
Synthetic peptide within human adx aa 51-100 / 184.
Host
Rabbit
Positive Control
SK-Br-3 cell lysate, K-562 cell lysate, HepG2 cell lysate, 293T cell lysate, Rat liver tissue lysate, Rat testis tissue lysate, Rat kidney tissue lysate, Mouse liver tissue lysate, PC-12, human placenta tissue, rat adrenal gland tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE63-56
RRID
APPLICATION DILUTION
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WB
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1:500-1:1,000
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IHC-P
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1:200-1:1,000
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IF-Cell
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1:50
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 19 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
ADX
SYNONYMS
Adrenal ferredoxin antibody;Adrenodoxin antibody;Adrenodoxin, mitochondrial antibody;ADX antibody;ADX_HUMAN antibody;FDX antibody;fdx1 antibody;Ferredoxin 1 antibody;Ferredoxin-1 antibody;Hepatoredoxin antibody;LOH11CR1D antibody;Mitochondrial adrenodoxin antibody;mitochondrial antibody
SUBCELLULAR LOCATION
Mitochondrion matrix.
FUNCTION
Adrenal ferredoxin (also adrenodoxin (ADX), adrenodoxin, mitochondrial, hepatoredoxin, ferredoxin-1 (FDX1)) is a protein that in humans is encoded by the FDX1 gene. In addition to the expressed gene at this chromosomal locus (11q22), there are pseudogenes located on chromosomes 20 and 21. Adrenodoxin is a small iron-sulfur protein that can accept and carry a single electron. Adrenodoxin functions as an electron transfer protein in the mitochondrial cytochrome P450 systems. The first enzyme in this system is adrenodoxin reductase that carries an FAD. FAD can be reduced by two electrons donated from coenzyme NADPH. These two electrons are transferred one a time to adrenodoxin. Adrenodoxin in return reduces mitochondrial cytochrome P450. This particular oxidation/reduction system is involved in the synthesis of steroid hormones in steroidogenic tissues. In addition, similar systems also function in vitamin D and bile acid synthesis in the kidney and liver respectively. Adrenodoxin has been identified in a number of different tissues but all forms have been shown to be identical and are not tissue specific.
CITATIONS
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Hu Jiahe,et al
Engineered-Doping Strategy for Self-Sufficient Reactive Oxygen Species Blossom to Amplify Ferroptosis/Cuproptosis Sensibilization in Hepatocellular Carcinoma Treatment
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WB
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Human
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Citation
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Caili Chen,et al
Bovine serum albumin-bound homologous targeted nanoparticles for breast cancer combinatorial therapy
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WB
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Mouse
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Citation
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