Adipose Triglyceride Lipase Recombinant Rabbit Monoclonal Antibody [JE37-49] (HA721951)
Catalog# HA721951
Rabbit Monoclonal to Adipose Triglyceride Lipase
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WB
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IHC-P
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IF-Tissue
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Human
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Mouse
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Rat
Lane 1: SiHa cell lysate (30 µg/Lane)
Lane 2: HepG2 cell lysate (30 µg/Lane)
Lane 3: HEK-293 cell lysate (30 µg/Lane)
Lane 4: A431 cell lysate (30 µg/Lane)
Lane 5: Mouse heart tissue lysate (40 µg/Lane)
Lane 6: Rat heart tissue lysate (40 µg/Lane)
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: Lane 1-4: 3 minutes; Lane 5-6: 5 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721951) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Adipose Triglyceride Lipase Recombinant Rabbit Monoclonal Antibody [JE37-49] (HA721951)
Immunogen
Recombinant protein within human adipose triglyceride lipase aa 330-450 / 504.
Host
Rabbit
Positive Control
SiHa cell lysate, HepG2 cell lysate, HEK-293 cell lysate, A431 cell lysate, Mouse heart tissue lysate, Rat heart tissue lysate, Mouse liver tissue lysate, Mouse white adipose tissue lysate, Mouse brown adipose tissue lysate, Rat brown adipose tissue lysate, mouse brown adipose tissue, rat breast tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE37-49
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IHC-P
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1:500-1:2,000
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IF-Tissue
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1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 55 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Adipose Triglyceride Lipase
SYNONYMS
1110001C14Rik antibody;Adipose triglyceride lipase antibody;ATGL antibody;ATGL DESNUTRIN antibody;Calcium independent phospholipase A2 antibody;Calcium-independent phospholipase A2 antibody;Desnutrin antibody;EC 3.1.1.3 antibody;FP17548 antibody;IPLA2 zeta antibody;IPLA2-zeta antibody;Mutant patatin like phospholipase domain containing 2 antibody;Patatin like phospholipase domain containing 2 antibody;PATATIN LIKE PHOSPHOLIPASE DOMAIN CONTAINING PROTEIN 2 antibody;Patatin-like phospholipase domain-containing protein 2 antibody;PEDF R antibody;PHOSPHOLIPASE A2 CALCIUM INDEPENDENT ZETA antibody;Pigment epithelium derived factor antibody;Pigment epithelium-derived factor antibody;plpl antibody;plpl2 antibody;PLPL2_HUMAN antibody;Pnpla2 antibody;Transport secretion protein 2 antibody;Transport secretion protein 2.2 antibody;Transport-secretion protein 2 antibody;Triglyceride hydrolase antibody;TTS 2.2 antibody;TTS2 antibody;TTS2.2 antibody;ZETA antibody
SUBCELLULAR LOCATION
Lipid droplet, Cell membrane, Cytoplasm.
FUNCTION
Adipose triglyceride lipase, also known as patatin-like phospholipase domain-containing protein 2 and ATGL, is an enzyme that in humans is encoded by the PNPLA2 gene. ATGL catalyses the first reaction of lipolysis, where triacylglycerols are hydrolysed to diacylglycerols. ATGL has very high substrate specificity for triacylglycerols. It contains a catalytic dyad using serine-aspartic acid. ATGL catalyses the first reaction of lipolysis. It hydrolysis triacylglycerols to diacylglycerols by attacking the fatty acid attached to carbon-3 of glycerol. ATGL acts as a control mechanism of lipolysis, as variations in diacylglycerol concentration impact enzymes in later stages of lipolysis.
CITATIONS
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"Regulation of a High-Iron Diet on Lipid Metabolism and Gut Microbiota in Mice"
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WB
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Mouse
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Citation
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