ADAMTS5 Recombinant Rabbit Monoclonal Antibody [PSH03-52] (HA722011)
Catalog# HA722011
Rabbit Monoclonal to ADAMTS5
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WB
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IF-Cell
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FC
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Human
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Mouse
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Rat
Lane 1: HepG2 cell lysate
Lane 2: LO2 cell lysate
Lane 3: Huh7 cell lysate
Lane 4: HeLa cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: PC-12 cell lysate
Lane 7: Human liver tissue lysate
Lane 8: Mouse placenta tissue lysate
Lane 9: Mouse uterus tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 102 kDa
Observed band size: 75 kDa
Exposure time: 50 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722011) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
ADAMTS5 Recombinant Rabbit Monoclonal Antibody [PSH03-52] (HA722011)
Immunogen
Recombinant protein within human adamts5 aa 251-500 / 930.
Host
Rabbit
Positive Control
HepG2 cell lysate, LO2 cell lysate, Huh7 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, human liver tissue lysate, mouse placenta tissue lysate, mouse uterus tissue lysate, HepG2, NIH/3T3.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
PSH03-52
RRID
APPLICATION DILUTION
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WB
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1:2,000
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IF-Cell
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1:100-1:250
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 102 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
ADAMTS5
SYNONYMS
A disintegrin and metalloproteinase with thrombospondin motifs 11 antibody;A disintegrin and metalloproteinase with thrombospondin motifs 5 antibody;A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 5 antibody;A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase 2) antibody;A disintegrin-like and metalloprotease with thrombospondin type 1 motif, 5 antibody;A Disintigrin And Metalloproteinase with ThromboSpondin motif-5 antibody;ADAM metallopeptidase with thrombospondin type 1 motif 5 antibody;ADAM TS 11 antibody;ADAM TS 5 antibody;ADAM TS5 antibody;ADAM-TS 11 antibody;ADAM-TS 5 antibody;ADAM-TS5 antibody;ADAMTS 11 antibody;ADAMTS 5 antibody;ADAMTS-11 antibody;ADAMTS-5 antibody;ADAMTS11 antibody;ADAMTS11, formerly antibody;Adamts5 antibody;ADMP 2 antibody;ADMP-2 antibody;ADMP2 antibody;Aggrecanase 2 antibody;Aggrecanase-2 antibody;ATS5_HUMAN antibody;FLJ36738 antibody;Implantin antibody;ThromboSpondin motif-5 antibody
SUBCELLULAR LOCATION
Secreted, extracellular space, extracellular matrix.
FUNCTION
A disintegrin and metalloproteinase with thrombospondin motifs 5 also known as ADAMTS5 is an enzyme that in humans is encoded by the ADAMTS5 gene. ADAMTS5 is a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene contains two C-terminal TS motifs and functions as aggrecanase to cleave aggrecan, a major proteoglycan of cartilage. ADAMTS5 may also have a role in the pathogenesis of human osteoarthritis.
CITATIONS
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Wenkai Li ,et al
A study of YangHe Decoction delaying the progression of osteoarthritis by inhibiting chondrocyte ferroptosis through the P53/SLC7A11/GPX4 signaling axis
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IHC
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Mouse
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Citation
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Ji Zhongyin,et al
Injectable hydrogel encapsulating siMMP13 with anti-ROS and anti-apoptotic functions for osteoarthritis treatment
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WB
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Mouse
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Citation
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