PRODUCT CODE: ET1703-60

ADAM10 Recombinant Rabbit Monoclonal Antibody [JM32-11] (ET1703-60)

  • Recombinant

Applications

  • WB

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of ADAM10 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-60, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Line 1: Jurkat cell lysate<br />
Line 2: Hela cell lysate
  • Western blot analysis of ADAM10 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-60, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Line 1: Jurkat cell lysate<br />
Line 2: Hela cell lysate
Western blot analysis of ADAM10 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-60, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Line 1: Jurkat cell lysate
Line 2: Hela cell lysate

Applications

  • WB

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

ADAM10 Recombinant Rabbit Monoclonal Antibody [JM32-11] (ET1703-60)

Immunogen

Synthetic peptide within human adam10 aa 715-748 / 748.

Host

Rabbit

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM32-11

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

84 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

ADAM10

SYNONYMS

A disintegrin and metalloprotease domain 10 antibody; A disintegrin and metalloproteinase domain 10 antibody; AD 10 antibody; AD10 antibody; AD18 antibody; ADA10_HUMAN antibody; ADAM 10 antibody; ADAM metallopeptidase domain 10 antibody; ADAM10 antibody; CD 156c antibody; CD156c antibody; CD156c antigen antibody; CDw156 antibody; disintegrin and metalloproteinase domain containing protein 10 antibody; Disintegrin and metalloproteinase domain-containing protein 10 antibody; HsT 18717 antibody; HsT18717 antibody; Kuz antibody; Kuzbanian antibody; Kuzbanian protein homolog antibody; Kuzbanian, Drosophila, homolog of antibody; MADM antibody; Mammalian disintegrin metalloprotease antibody; Mammalian disintegrin-metalloprotease antibody; RAK antibody

TISSUE SPECIFICITY

Expressed in the brain (at protein level). Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver.

POST-TRANSLATIONAL MODIFICATION

The precursor is cleaved by furin and PCSK7.

SUBCELLULAR LOCATION

Cell membrane. Endomembrane system.

FUNCTION

ADAM (a disintegrin and metalloprotease) proteins are a family of over 30 membrane-anchored, glycosylated, Zn2+ dependent proteases that are involved in cell-cell, cell-matrix interface related processes including fertilization, muscle fusion, secretion of TNFα (tumor necrosis factor α), and modulation of the neurogenic function of Notch and Delta. ADAM proteins possess a signal-domain, a pro-domain, a metalloprotease domain, a disintegrin domain (integrin ligand) a cysteine-rich region, an epidermal growth factor-like domain, a transmembrane domain and a cytoplasmic tail. ADAMs are expressed in brain, testis, epididymis, ovary, breast, placenta, liver, heart, lung, bone and muscle, and catalyze proteolysis, adhesion, fusion and intracellular signaling. ADAM10 is a TNF-processing enzyme that cleaves pro-TNF, a membrane-bound precusor protein, at Ala 76-Val 77, which causes membrane shedding of soluble TNF.